MAB17291-100

antibody from Novus Biologicals

Targeting: TREM2 TREM-2, Trem2a, Trem2b, Trem2c

Immunocytochemistry

Antibody data

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Validation data

Product number: MAB17291-100 - Provider product page
Provider: Novus Biologicals
Product name: Rat Monoclonal TREM2 Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from hybridoma culture supernatant. Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.
Reactivity: Human, Mouse
Host: Rat
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

TREM2 protein structure - MAB17291-100 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of TREM-2 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of TREM-2 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of TREM-2 in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM-2 or Human TREM-1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM-2 or (B) human TREM-1 was stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0061).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of TREM-2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂ Secondary Antibody (Catalog # F0105B).