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- References [9]
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- Product number
- MAB17291-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse TREM2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q99NH8- Isotype
- IgG
- Antibody clone number
- 237920
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model.
Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome.
Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism.
Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis.
Interleukin-2 improves amyloid pathology, synaptic failure and memory in Alzheimer's disease mice.
A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells.
TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.
Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke.
Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease.
Zhong L, Xu Y, Zhuo R, Wang T, Wang K, Huang R, Wang D, Gao Y, Zhu Y, Sheng X, Chen K, Wang N, Zhu L, Can D, Marten Y, Shinohara M, Liu CC, Du D, Sun H, Wen L, Xu H, Bu G, Chen XF
Nature communications 2019 Mar 25;10(1):1365
Nature communications 2019 Mar 25;10(1):1365
Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome.
Russo MA, Fiore NT, van Vreden C, Bailey D, Santarelli DM, McGuire HM, Fazekas de St Groth B, Austin PJ
Journal of neuroinflammation 2019 Mar 18;16(1):63
Journal of neuroinflammation 2019 Mar 18;16(1):63
Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism.
Götzl JK, Brendel M, Werner G, Parhizkar S, Sebastian Monasor L, Kleinberger G, Colombo AV, Deussing M, Wagner M, Winkelmann J, Diehl-Schmid J, Levin J, Fellerer K, Reifschneider A, Bultmann S, Bartenstein P, Rominger A, Tahirovic S, Smith ST, Madore C, Butovsky O, Capell A, Haass C
EMBO molecular medicine 2019 Jun;11(6)
EMBO molecular medicine 2019 Jun;11(6)
Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis.
Garcia-Reitboeck P, Phillips A, Piers TM, Villegas-Llerena C, Butler M, Mallach A, Rodrigues C, Arber CE, Heslegrave A, Zetterberg H, Neumann H, Neame S, Houlden H, Hardy J, Pocock JM
Cell reports 2018 Aug 28;24(9):2300-2311
Cell reports 2018 Aug 28;24(9):2300-2311
Interleukin-2 improves amyloid pathology, synaptic failure and memory in Alzheimer's disease mice.
Alves S, Churlaud G, Audrain M, Michaelsen-Preusse K, Fol R, Souchet B, Braudeau J, Korte M, Klatzmann D, Cartier N
Brain : a journal of neurology 2017 Mar 1;140(3):826-842
Brain : a journal of neurology 2017 Mar 1;140(3):826-842
A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells.
Varnum MM, Clayton KA, Yoshii-Kitahara A, Yonemoto G, Koro L, Ikezu S, Ikezu T
The Journal of biological chemistry 2017 Jun 23;292(25):10651-10663
The Journal of biological chemistry 2017 Jun 23;292(25):10651-10663
TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.
Wu K, Byers DE, Jin X, Agapov E, Alexander-Brett J, Patel AC, Cella M, Gilfilan S, Colonna M, Kober DL, Brett TJ, Holtzman MJ
The Journal of experimental medicine 2015 May 4;212(5):681-97
The Journal of experimental medicine 2015 May 4;212(5):681-97
Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke.
Kawabori M, Kacimi R, Kauppinen T, Calosing C, Kim JY, Hsieh CL, Nakamura MC, Yenari MA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 25;35(8):3384-96
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 25;35(8):3384-96
Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease.
Savage JC, Jay T, Goduni E, Quigley C, Mariani MM, Malm T, Ransohoff RM, Lamb BT, Landreth GE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 22;35(16):6532-43
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 22;35(16):6532-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TREM-2 in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM-2 or Human TREM-1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM-2 or (B) human TREM-1 was stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0061).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TREM-2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TREM-2 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TREM-2 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM-2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
