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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-18763 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TREM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues.
Satoh J, Kawana N, Yamamoto Y, Ishida T, Saito Y, Arima K
Alzheimer's research & therapy 2013;5(4):30
Alzheimer's research & therapy 2013;5(4):30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TREM2 using TREM2 Polyclonal Antibody (Product # PA5-18763) in paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TREM2 using TREM2 Polyclonal Antibody (Product # PA5-18763) in paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TREM2 in human spleen, macrophages. Samples were incubated with TREM2 polyclonal antibody (Product # PA5-18763) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (PFA fixed) analysis of TREM2 using TREM2 Polyclonal Antibody (Product # PA5-18763) (2 µg/mL) in staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of TREM2 using TREM2 Polyclonal Antibody (Product # PA5-18763) in paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 The specificity of anti-TREM2 antibodies . The specificity of seven commercially available anti-TREM2 antibodies was determined by Western blot analysis of recombinant human TREM2 protein expressed in HEK293 cells following transfection of the expression vector. The upper panels represent (a) HPA010917 (at the final concentration of 200 ng/mL), (b) AF1828 (100 ng/mL), (c) 2B5 (1 mug/mL), (d) bs-2723R (2 mug/mL), (e) ab69405 (400 ng/mL), (f) ab85851 (500 ng/mL), and (g) PA5-18763 (500 ng/mL). The lower panels indicate the corresponding blots of the upper panels with an antibody against HSP60, an internal control of protein loading. The lanes represent the protein extract (80 mug each) of (1) nontransfected and (2) transfected HEK293 cells. The position of TREM2 is indicated by an arrowhead.
