PA5-18763

antibody from Invitrogen Antibodies

Targeting: TREM2 TREM-2, Trem2a, Trem2b, Trem2c

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18763

Provider

Invitrogen Antibodies

Product name

TREM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues.

Satoh J, Kawana N, Yamamoto Y, Ishida T, Saito Y, Arima K
Alzheimer's research & therapy 2013;5(4):30

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 The specificity of anti-TREM2 antibodies . The specificity of seven commercially available anti-TREM2 antibodies was determined by Western blot analysis of recombinant human TREM2 protein expressed in HEK293 cells following transfection of the expression vector. The upper panels represent (a) HPA010917 (at the final concentration of 200 ng/mL), (b) AF1828 (100 ng/mL), (c) 2B5 (1 mug/mL), (d) bs-2723R (2 mug/mL), (e) ab69405 (400 ng/mL), (f) ab85851 (500 ng/mL), and (g) PA5-18763 (500 ng/mL). The lower panels indicate the corresponding blots of the upper panels with an antibody against HSP60, an internal control of protein loading. The lanes represent the protein extract (80 mug each) of (1) nontransfected and (2) transfected HEK293 cells. The position of TREM2 is indicated by an arrowhead.