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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN359012 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-STIP1 Homology and U-Box Containing Protein 1 (STUB1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the C-term of human CHIP (STUB1).
- Description
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator.
An androgen receptor NH2-terminal conserved motif interacts with the COOH terminus of the Hsp70-interacting protein (CHIP).
CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription.
CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival.
Ubiquitylation of neuronal nitric-oxide synthase by CHIP, a chaperone-dependent E3 ligase.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation.
ErbB2 degradation mediated by the co-chaperone protein CHIP.
CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity.
Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions.
Alberti S, Böhse K, Arndt V, Schmitz A, Höhfeld J
Molecular biology of the cell 2004 Sep;15(9):4003-10
Molecular biology of the cell 2004 Sep;15(9):4003-10
An androgen receptor NH2-terminal conserved motif interacts with the COOH terminus of the Hsp70-interacting protein (CHIP).
He B, Bai S, Hnat AT, Kalman RI, Minges JT, Patterson C, Wilson EM
The Journal of biological chemistry 2004 Jul 16;279(29):30643-53
The Journal of biological chemistry 2004 Jul 16;279(29):30643-53
CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription.
Li L, Xin H, Xu X, Huang M, Zhang X, Chen Y, Zhang S, Fu XY, Chang Z
Molecular and cellular biology 2004 Jan;24(2):856-64
Molecular and cellular biology 2004 Jan;24(2):856-64
CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival.
Shimura H, Schwartz D, Gygi SP, Kosik KS
The Journal of biological chemistry 2004 Feb 6;279(6):4869-76
The Journal of biological chemistry 2004 Feb 6;279(6):4869-76
Ubiquitylation of neuronal nitric-oxide synthase by CHIP, a chaperone-dependent E3 ligase.
Peng HM, Morishima Y, Jenkins GJ, Dunbar AY, Lau M, Patterson C, Pratt WB, Osawa Y
The Journal of biological chemistry 2004 Dec 17;279(51):52970-7
The Journal of biological chemistry 2004 Dec 17;279(51):52970-7
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation.
Petrucelli L, Dickson D, Kehoe K, Taylor J, Snyder H, Grover A, De Lucia M, McGowan E, Lewis J, Prihar G, Kim J, Dillmann WH, Browne SE, Hall A, Voellmy R, Tsuboi Y, Dawson TM, Wolozin B, Hardy J, Hutton M
Human molecular genetics 2004 Apr 1;13(7):703-14
Human molecular genetics 2004 Apr 1;13(7):703-14
ErbB2 degradation mediated by the co-chaperone protein CHIP.
Zhou P, Fernandes N, Dodge IL, Reddi AL, Rao N, Safran H, DiPetrillo TA, Wazer DE, Band V, Band H
The Journal of biological chemistry 2003 Apr 18;278(16):13829-37
The Journal of biological chemistry 2003 Apr 18;278(16):13829-37
CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity.
Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R
Molecular cell 2002 Jul;10(1):55-67
Molecular cell 2002 Jul;10(1):55-67
Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions.
Ballinger CA, Connell P, Wu Y, Hu Z, Thompson LJ, Yin LY, Patterson C
Molecular and cellular biology 1999 Jun;19(6):4535-45
Molecular and cellular biology 1999 Jun;19(6):4535-45
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-CHIP (STUB1) Antibody (C-term) in Ramos cell line lysates (35ug/lane). CHIP (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- (LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
