PA5-18011
antibody from Invitrogen Antibodies
Targeting: STUB1
CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-18011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STUB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Muscle lysate using Product # PA5-18011 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STUB1 by a STUB1 monoclonal antibody (Product # PA5-18011) at a concentration of 0.3 µg/mL. Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-E3 ubiquitin-protein ligase CHIP (STUB1) Polyclonal Antibody (Product # PA5-18011) on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2), HeLa (Lane 3), LNCaP (Lane 4), Jurkat (Lane 5) and Hep G2 (Lane 6) and 35 kDa band corresponding to STUB1 was observed except in Jurkat and Hep G2 as reported in literature. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of STUB1 in Human Small Intestine using a STUB1 monoclonal antibody (Product #PA5-18011) at 2.5 µg/mL. The Human Small Intestine tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.