PA5-29024
antibody from Invitrogen Antibodies
Targeting: STUB1
CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-29024 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STUB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using STUB1 Polyclonal Antibody (Product # PA5-29024). Sample (30 µg whole cell lysate). A: 293T. B: Hep G2. 10% SDS PAGE. STUB1 Polyclonal Antibody (Product # PA5-29024) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-E3 ubiquitin-protein ligase CHIP (STUB1) Polyclonal Antibody (Product # PA5-29024) on Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2), HeLa (Lane 3), LNCaP (Lane 4), Jurkat (Lane 5) and Hep G2 (Lane 6) and 35 kDa band corresponding to STUB1 was observed in HEK-293 and MCF7 only along with uncharacterized bands (*) at~60, ~25 kDa. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of STUB1 in paraformaldehyde-fixed HeLa cells using a STUB1 polyclonal antibody (Product # PA5-29024) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of STUB1 was performed in paraffin-embedded mouse esophagus tissue using STUB1 Polyclonal Antibody (Product # PA5-29024) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using STUB1 (Product # PA5-29024) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.