Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN954992 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-STIP1 Homology and U-Box Containing Protein 1 (STUB1) (C-Term), (AA 243-271) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of Human STUB1
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 243-271
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Promotion of CHIP-mediated p53 degradation protects the heart from ischemic injury.
CHIP participates in protein triage decisions by preferentially ubiquitinating Hsp70-bound substrates.
The ubiquitin ligase CHIP/STUB1 targets mutant keratins for degradation.
Naito AT, Okada S, Minamino T, Iwanaga K, Liu ML, Sumida T, Nomura S, Sahara N, Mizoroki T, Takashima A, Akazawa H, Nagai T, Shiojima I, Komuro I
Circulation research 2010 Jun 11;106(11):1692-702
Circulation research 2010 Jun 11;106(11):1692-702
CHIP participates in protein triage decisions by preferentially ubiquitinating Hsp70-bound substrates.
Stankiewicz M, Nikolay R, Rybin V, Mayer MP
The FEBS journal 2010 Aug;277(16):3353-67
The FEBS journal 2010 Aug;277(16):3353-67
The ubiquitin ligase CHIP/STUB1 targets mutant keratins for degradation.
Löffek S, Wöll S, Höhfeld J, Leube RE, Has C, Bruckner-Tuderman L, Magin TM
Human mutation 2010 Apr;31(4):466-76
Human mutation 2010 Apr;31(4):466-76
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of STUB1 / CHIP Antibody (C-term) Cat.-No AP54084PU-N in K562 cell line lysates (35ug/lane). This demonstrates the STUB1 antibody detected the STUB1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of K562 cells using STUB1 / CHIP Antibody (C-term) Cat.-No AP54084PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.