PA1-776

antibody from Invitrogen Antibodies

Targeting: DNAJC5 CLN4, DNAJC5A, FLJ00118, FLJ13070

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-776

Provider

Invitrogen Antibodies

Product name

CSP alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-776 detects Cysteine String Protein Alpha in mouse and rat brain samples. PA1-776 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~34 kDa protein representing Cysteine String Protein Alpha in rat and mouse brain homogenates. The PA1-776 immunogen is a synthetic peptide corresponding to residues E(180) T T Q L T A D S H P S Y H T D G F N(198) of rat CSP. This sequence is conserved across many species including mouse, human and bovine. This peptide (Cat. # PEP-287) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Cysteine String Protein alpha was performed using PC-12 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Cysteine String Protein alpha Rabbit Polyclonal Antibody (PA1-776, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..