PA1-776

antibody from Invitrogen Antibodies

Targeting: DNAJC5 CLN4, DNAJC5A, FLJ00118, FLJ13070

Antibody data

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Validation data

Product number: PA1-776 - Provider product page
Provider: Invitrogen Antibodies
Product name: CSP alpha Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-776 detects Cysteine String Protein Alpha in mouse and rat brain samples. PA1-776 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~34 kDa protein representing Cysteine String Protein Alpha in rat and mouse brain homogenates. The PA1-776 immunogen is a synthetic peptide corresponding to residues E(180) T T Q L T A D S H P S Y H T D G F N(198) of rat CSP. This sequence is conserved across many species including mouse, human and bovine. This peptide (Cat. # PEP-287) is available for use in neutralization and control experiments.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

DNAJC5 protein structure - PA1-776 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Cysteine String Protein Alpha in rat brain samples using Product # PA1-776.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cysteine String Protein alpha was performed using whole cell lysates of SH-SY5Y (Lane 1) and NIH/3T3 (Lane 2). The blots were probed with Anti- Cysteine String Protein alpha Rabbit polyclonal Antibody (Product # PA1-776, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Bands of ~ 35 and 26 kDa, corresponding to mature and immature forms of Cysteine String Protein alpha respectively, were observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CSP alpha was achieved by transfecting NIH/3T3 cells with CSP specific siRNAs (Silencer® select Product # s201218, s64547). Western blot analysis (Fig a) was performed using membrane enriched extracts from the CSP alpha knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti- CSP alpha Rabbit polyclonal Antibody (Product # PA1-776, 1µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CSP alpha.