TA321059

antibody from OriGene

Targeting: CST3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321059

Provider

OriGene

Product name

Anti-CST3 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 102-118 amino acids of Human cystatin C

Description

Anti-CST3 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.6mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 16 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human colon. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using CST3 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC