AF1238

antibody from R&D Systems

Targeting: CST3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1238

Provider

R&D Systems

Product name

Mouse Cystatin C Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse and human Cystatin C in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Cystatin D is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P21460


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution.

Masuda T, Sankowski R, Staszewski O, Böttcher C, Amann L, Sagar, Scheiwe C, Nessler S, Kunz P, van Loo G, Coenen VA, Reinacher PC, Michel A, Sure U, Gold R, Grün D, Priller J, Stadelmann C, Prinz M
Nature 2019 Feb;566(7744):388-392

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Megalin dependent urinary cystatin C excretion in ischemic kidney injury in rats.

Jensen D, Kierulf-Lassen C, Kristensen MLV, Nørregaard R, Weyer K, Nielsen R, Christensen EI, Birn H
PloS one 2017;12(6):e0178796

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Peripheral role of cathepsin S in Th1 cell-dependent transition of nerve injury-induced acute pain to a chronic pain state.

Zhang X, Wu Z, Hayashi Y, Okada R, Nakanishi H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 19;34(8):3013-22

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Urinary clusterin, cystatin C, beta2-microglobulin and total protein as markers to detect drug-induced kidney injury.

Dieterle F, Perentes E, Cordier A, Roth DR, Verdes P, Grenet O, Pantano S, Moulin P, Wahl D, Mahl A, End P, Staedtler F, Legay F, Carl K, Laurie D, Chibout SD, Vonderscher J, Maurer G
Nature biotechnology 2010 May;28(5):463-9

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse Cystatin C by Western Blot. Western blot shows lysates of mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cystatin C in Mouse Spleen. Cystatin C was detected in immersion fixed frozen sections of mouse spleen using Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.