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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA504494 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504494, RRID:AB_2622449
- Product name
- CST3 (Cystatin C) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Antibody type
- Monoclonal
- Description
- CST3 (Cystatin C) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CST3
- Isotype
- IgG
- Antibody clone number
- OTI1A6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST3 (RC210730, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CST3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-CST3 mouse monoclonal antibody (TA504494).
- Validation comment
- IF
