Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-19108 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cystatin C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cystatin C was performed following immunoprecipitation of Cystatin C-FLAG with an anti-FLAG antibody from ARPE-19 cells transfected with a construct encoding a FLAG-tagged Cystatin C. Following immunoprecipitation, proteins were transferred to a nitrocellulose membrane, blocked, and then probed with a Cystatin C polyclonal antibody (Product # PA5-19108) at a dilution of 1:500 for 1 hour at room temperature. The blot was washed, and probed with a DyLight 800-conjugated anti-goat IgG secondary antibody at a dilution of 1:10,000 for 40 minutes at room temperature. Detection was performed using a near IR imager. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cystatin C by a Cystatin C monoclonal antibody (Product # PA5-19108) at a concentration of 0.1 µg/mL. Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Testes lysate using Product # PA5-19108 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.