15235-1-AP

antibody from Proteintech Group

Targeting: SLC25A1 CTP, SLC20A3

Provider product page for 15235-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [46]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 15235-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15235-1-AP, RRID:AB_2254794
Product name: SLC25A1 antibody
Antibody type: Polyclonal
Description: KD/KO validated SLC25A1 antibody (Cat. #15235-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SLC25A1 protein structure - 15235-1-AP shown in red.

Submitted references SLC25A1-Mediated Cholesterol Accumulation Promotes Endometriosis Progression by Enhancing Endometrial Stromal Cell Proliferation, Invasion, and M2 Macrophage Polarization.

Yang P, Wang T, Miao Y, Liu W, Zhu Y, Sun J
International journal of biological sciences 2026;22(1):466-480

Citrate-functionalised hydroxyapatite mimics the microenvironment of bone metastases and demonstrates the role of citrate in prostate cancer colonization of bone.

Pasquarelli A, Cavassi E, Bucchi L, Boanini E, Pompo GD, Lipreri MV, Perut F, Bouidida Y, Sonveaux P, Rubini K, Montesissa M, Santonocito R, Baldini N, Avnet S
Acta biomaterialia 2026 Jun;216:520-540

Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes.

Wynne M, Zlatic SA, Park AS, Crocker A, Mendez-Vazquez H, Liporace E, Gokhale A, Tower-Gilchrist C, Robinette M, Purcell RH, Bassell GJ, Werner E, Kwong JQ, Faundez V
bioRxiv : the preprint server for biology 2026 Jan 9;

PRMT3-mediated post-translational adaptation to fasting regulates metabolic flexibility.

Huang Z, Liu X, Chen X, Zhou Y, Chen Q, Liu Y, Zhu H, Cheng K, Feng Y, Dong M, Song L, Wang L, Liu S, Shan T, Kuang S, Dong Y, Vidal-Puig A, Zhang Y, Jia Z
Nature communications 2026 Feb 2;17(1)

Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle.

Xie A, Brunner JS, Chakraborty S, Montero AM, Bridgeman AE, Paras KI, Cui R, Fagoaga-Eugui M, Komza M, Arnold PK, Jackson BT, Noriega Madrazo S, Atmane MI, Carrasco SE, Finley LWS
Cell 2026 Apr 30;189(9):2684-2699.e21

Human receptive endometrial assembloid for deciphering the implantation window.

Zhang Y, Zhao R, Yang C, Song J, Liu P, Li Y, Liu B, Li T, Yin C, Lu M, Hou Z, Zhang C, Chen ZJ, Wu K, Zhao H
eLife 2026 Apr 1;12

The integral membrane protein smim4 modulates redox balance via malate compartmentalization in pancreatic cancer.

Wang B, Han X, Lin X, Wang J, You C, Chen K, Chen Y, Meng F, Jiang H, Zheng F, Zhang Y, Lyu J, Bai Y, Qu X, Zhou D, Jiang M, Cui W, Lyu J, Fang H
Nature communications 2025 Nov 5;16(1):9772

SLC25A1 and ACLY maintain cytosolic acetyl-CoA and regulate ferroptosis susceptibility via FSP1 acetylation.

Li W, Han J, Huang B, Xu T, Wan Y, Luo D, Kong W, Yu Y, Zhang L, Nian Y, Chu B, Yin C
The EMBO journal 2025 Mar;44(6):1641-1662

DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway.

Shi L, Fei X, Huang J, He B, Sun Z, Sun G
Journal of translational medicine 2025 Mar 24;23(1):363

Induction of Fructose Mediated De Novo Lipogenesis Coexists with the Upregulation of Mitochondrial Oxidative Function in Mice Livers.

Bhattacharjee P, Fadlaoui A, Ryan CE, Carlson CB, Zhang D, Sunny NE
The Journal of nutrition 2025 Jun;155(6):1768-1781

Enhanced LDL uptake and PPARα signaling support OSCC cell survival under glutamine deprivation.

Cai L, Chen Y, Tang S, Wang Q, Xu Y, Pan Y, Yang F, Chen T, Chen Q, Zhou Y, Shen YQ
Medical oncology (Northwood, London, England) 2025 Jul 14;42(8):332

A hierarchical hepatic de novo lipogenesis substrate supply network utilizing pyruvate, acetate, and ketones.

Rauckhorst AJ, Sheldon RD, Pape DJ, Ahmed A, Falls-Hubert KC, Merrill RA, Brown RF, Deshmukh K, Vallim TA, Deja S, Burgess SC, Taylor EB
Cell metabolism 2025 Jan 7;37(1):255-273.e6

Sodium butyrate regulates macrophage polarization by TGR5/β-arrestin2 in vitro.

Liu M, Xie WJ, Zhang X, Wu W, Li G, Wang L
Molecular medicine (Cambridge, Mass.) 2025 Jan 29;31(1):31

The oxygen level in air directs airway epithelial cell differentiation by controlling mitochondrial citrate export.

Kim BR, Rauckhorst AJ, Chimenti MS, Rehman T, Keen HL, Karp PH, Taylor EB, Welsh MJ
Science advances 2025 Jan 24;11(4):eadr2282

Lipin-1 restrains macrophage lipid synthesis to promote inflammation resolution.

Bamgbose TT, Schilke RM, Igiehon OO, Nkadi EH, Binwal M, Custis D, Bharrhan S, Schwarz B, Bohrnsen E, Bosio CM, Scott RS, Yurdagul A Jr, Finck BN, Woolard MD
Journal of immunology (Baltimore, Md. : 1950) 2025 Jan 1;214(1):85-103

SLC25A1 promotes lymph node metastasis of esophageal squamous cell carcinoma by regulating lipid metabolism.

Zhang G, Wu J, Ji M, Liu X, Shi M
International journal of oncology 2025 Feb;66(2)

Angiotensin-(1-7) Modulates the Warburg Effect to Alleviate Inflammation in LPS-Induced Macrophages and Septic Mice.

Yu D, Huang W, Sheng M, Zhang S, Pan H, Ren F, Luo L, Zhou J, Huang D, Tang L
Journal of inflammation research 2024;17:469-485

The mitochondrial citrate carrier SLC25A1 regulates metabolic reprogramming and morphogenesis in the developing heart.

Ohanele C, Peoples JN, Karlstaedt A, Geiger JT, Gayle AD, Ghazal N, Sohani F, Brown ME, Davis ME, Porter GA Jr, Faundez V, Kwong JQ
Communications biology 2024 Oct 31;7(1):1422

Stimulation of neutral lipid synthesis via viral growth factor signaling and ATP citrate lyase during vaccinia virus infection.

Pant A, Brahim Belhaouari D, Dsouza L, Yang Z
Journal of virology 2024 Nov 19;98(11):e0110324

Loss of the mitochondrial carrier, SLC25A1, during embryogenesis induces a unique senescence program controlled by p53.

Kasprzyk-Pawelec A, Tan M, Rahhal R, McIntosh A, Fernandez H, Mosaoa R, Jiang L, Pearson GW, Glasgow E, Vockley J, Albanese C, Avantaggiati ML
bioRxiv : the preprint server for biology 2024 May 31;

Mitochondrial citrate carrier SLC25A1 is a dosage-dependent regulator of metabolic reprogramming and morphogenesis in the developing heart.

Ohanele C, Peoples JN, Karlstaedt A, Geiger JT, Gayle AD, Ghazal N, Sohani F, Brown ME, Davis ME, Porter GA, Faundez V, Kwong JQ
bioRxiv : the preprint server for biology 2024 May 13;

Identification of MYC synthetic lethal genes and networks.

Martin TD, Choi MY, Patel R, Liang A, Li MZ, Elledge SJ
bioRxiv : the preprint server for biology 2024 Apr 25;

The citrate transporters SLC13A5 and SLC25A1 elicit different metabolic responses and phenotypes in the mouse.

Fernandez-Fuente G, Overmyer KA, Lawton AJ, Kasza I, Shapiro SL, Gallego-Muñoz P, Coon JJ, Denu JM, Alexander CM, Puglielli L
Communications biology 2023 Sep 9;6(1):926

A novel SLC25A1 inhibitor, parthenolide, suppresses the growth and stemness of liver cancer stem cells with metabolic vulnerability.

Zhang Z, Qiao Y, Sun Q, Peng L, Sun L
Cell death discovery 2023 Sep 23;9(1):350

Systemic Proteome Phenotypes Reveal Defective Metabolic Flexibility in Mecp2 Mutants.

Zlatic SA, Werner E, Surapaneni V, Lee CE, Gokhale A, Singleton K, Duong D, Crocker A, Gentile K, Middleton F, Dalloul JM, Liu WL, Patgiri A, Tarquinio D, Carpenter R, Faundez V
bioRxiv : the preprint server for biology 2023 Sep 1;

Lipin-1 restrains macrophage lipid synthesis to promote inflammation resolution.

Bamgbose TT, Schilke RM, Igiehon OO, Nkadi EH, Custis D, Bharrhan S, Schwarz B, Bohrnsen E, Bosio CM, Scott RS, Yurdagul A Jr, Finck BN, Woolard MD
bioRxiv : the preprint server for biology 2023 Oct 25;

A comprehensive analysis of SLC25A1 expression and its oncogenic role in pan-cancer.

You X, Huang L, Huang O, Deng Y, Shi X
Discover oncology 2023 Nov 19;14(1):207

Mitochondrial citrate metabolism and efflux regulate BeWo differentiation.

Mahr RM, Jena S, Nashif SK, Nelson AB, Rauckhorst AJ, Rome FI, Sheldon RD, Hughey CC, Puchalska P, Gearhart MD, Taylor EB, Crawford PA, Wernimont SA
Scientific reports 2023 May 6;13(1):7387

Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism.

Landowski M, Bhute VJ, Grindel S, Haugstad Z, Gyening YK, Tytanic M, Brush RS, Moyer LJ, Nelson DW, Davis CR, Yen CE, Ikeda S, Agbaga MP, Ikeda A
Communications biology 2023 Jan 4;6(1):8

Mitochondrial citrate metabolism and efflux regulates trophoblast differentiation.

Mahr RM, Jena S, Nashif SK, Nelson AB, Rauckhorst AJ, Rome FI, Sheldon RD, Hughey CC, Puchalska P, Gearhart MD, Taylor EB, Crawford PA, Wernimont SA
bioRxiv : the preprint server for biology 2023 Jan 22;

Bempedoic Acid Unveils Therapeutic Potential in Non-Alcoholic Fatty Liver Disease: Suppression of the Hepatic PXR-SLC13A5/ACLY Signaling Axis.

Sun Q, Guo Y, Hu W, Zhang M, Wang S, Lei Y, Meng H, Li N, Xu P, Li Z, Lin H, Huang F, Qiu Z
Drug metabolism and disposition: the biological fate of chemicals 2023 Dec;51(12):1628-1641

Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants.

Zlatic SA, Werner E, Surapaneni V, Lee CE, Gokhale A, Singleton K, Duong D, Crocker A, Gentile K, Middleton F, Dalloul JM, Liu WL, Patgiri A, Tarquinio D, Carpenter R, Faundez V
Human molecular genetics 2023 Dec 12;33(1):12-32

IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.

Liu W, Zhou H, Wang H, Zhang Q, Zhang R, Willard B, Liu C, Kang Z, Li X, Li X
Nature communications 2022 May 18;13(1):2748

A non-canonical tricarboxylic acid cycle underlies cellular identity.

Arnold PK, Jackson BT, Paras KI, Brunner JS, Hart ML, Newsom OJ, Alibeckoff SP, Endress J, Drill E, Sullivan LB, Finley LWS
Nature 2022 Mar;603(7901):477-481

Hypoxia promotes osteogenesis by facilitating acetyl-CoA-mediated mitochondrial-nuclear communication.

Pouikli A, Maleszewska M, Parekh S, Yang M, Nikopoulou C, Bonfiglio JJ, Mylonas C, Sandoval T, Schumacher AL, Hinze Y, Matic I, Frezza C, Tessarz P
The EMBO journal 2022 Dec 1;41(23):e111239

SLC25A1 promotes tumor growth and survival by reprogramming energy metabolism in colorectal cancer.

Yang Y, He J, Zhang B, Zhang Z, Jia G, Liu S, Wu T, He X, Wang N
Cell death & disease 2021 Nov 27;12(12):1108

Mitochondrial Efflux of Citrate and Isocitrate Is Fully Dispensable for Glucose-Stimulated Insulin Secretion and Pancreatic Islet β-Cell Function.

Bauchle CJ, Rohli KE, Boyer CK, Pal V, Rocheleau JV, Liu S, Imai Y, Taylor EB, Stephens SB
Diabetes 2021 Aug;70(8):1717-1728

Regulation on Citrate Influx and Metabolism through Inhibiting SLC13A5 and ACLY: A Novel Mechanism Mediating the Therapeutic Effects of Curcumin on NAFLD.

Sun Q, Niu Q, Guo Y, Zhuang Y, Li X, Liu J, Li N, Li Z, Huang F, Qiu Z
Journal of agricultural and food chemistry 2021 Aug 11;69(31):8714-8725

Inhibition of the mitochondrial citrate carrier, Slc25a1, reverts steatosis, glucose intolerance, and inflammation in preclinical models of NAFLD/NASH.

Tan M, Mosaoa R, Graham GT, Kasprzyk-Pawelec A, Gadre S, Parasido E, Catalina-Rodriguez O, Foley P, Giaccone G, Cheema A, Kallakury B, Albanese C, Yi C, Avantaggiati ML
Cell death and differentiation 2020 Jul;27(7):2143-2157

Long non-coding RNA BRM promotes proliferation and invasion of papillary thyroid carcinoma by regulating the microRNA-331-3p/SLC25A1 axis.

Liu S, Zhang D, Chen L, Gao S, Huang X
Oncology letters 2020 Apr;19(4):3071-3078

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Schwörer S, Berisa M, Violante S, Qin W, Zhu J, Hendrickson RC, Cross JR, Thompson CB
The EMBO journal 2020 Apr 15;39(8):e103334

A novel isoform of myosin 18A (Myo18Aγ) is an essential sarcomeric protein in mouse heart.

Horsthemke M, Nutter LMJ, Bachg AC, Skryabin BV, Honnert U, Zobel T, Bogdan S, Stoll M, Seidl MD, Müller FU, Ravens U, Unger A, Linke WA, van Gorp PRR, de Vries AAF, Bähler M, Hanley PJ
The Journal of biological chemistry 2019 May 3;294(18):7202-7218

Systems Analysis of the 22q11.2 Microdeletion Syndrome Converges on a Mitochondrial Interactome Necessary for Synapse Function and Behavior.

Gokhale A, Hartwig C, Freeman AAH, Bassell JL, Zlatic SA, Sapp Savas C, Vadlamudi T, Abudulai F, Pham TT, Crocker A, Werner E, Wen Z, Repetto GM, Gogos JA, Claypool SM, Forsyth JK, Bearden CE, Glausier J, Lewis DA, Seyfried NT, Kwong JQ, Faundez V
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 May 1;39(18):3561-3581

Glycolytic Switch Is Required for Transdifferentiation to Endothelial Lineage.

Lai L, Reineke E, Hamilton DJ, Cooke JP
Circulation 2019 Jan 2;139(1):119-133

An overview of combined D-2- and L-2-hydroxyglutaric aciduria: functional analysis of CIC variants.

Pop A, Williams M, Struys EA, Monné M, Jansen EEW, De Grassi A, Kanhai WA, Scarcia P, Ojeda MRF, Porcelli V, van Dooren SJM, Lennertz P, Nota B, Abdenur JE, Coman D, Das AM, El-Gharbawy A, Nuoffer JM, Polic B, Santer R, Weinhold N, Zuccarelli B, Palmieri F, Palmieri L, Salomons GS
Journal of inherited metabolic disease 2018 Mar;41(2):169-180

Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.

Nota B, Struys EA, Pop A, Jansen EE, Fernandez Ojeda MR, Kanhai WA, Kranendijk M, van Dooren SJ, Bevova MR, Sistermans EA, Nieuwint AW, Barth M, Ben-Omran T, Hoffmann GF, de Lonlay P, McDonald MT, Meberg A, Muntau AC, Nuoffer JM, Parini R, Read MH, Renneberg A, Santer R, Strahleck T, van Schaftingen E, van der Knaap MS, Jakobs C, Salomons GS
American journal of human genetics 2013 Apr 4;92(4):627-31

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15235-1-AP(SLC25A1 antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 15235-1-AP(SLC25A1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The SLC25A1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SLC25A1. This antibody recognizes human, mouse, rat antigen. The SLC25A1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 15235-1-AP.pdf

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