Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-85163 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (88%), Pig (99%), Rhesus Monkey (100%), Bovine (97%). Positive Control: Huh-7, HepG2, mouse cerebellum, rat liver. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A1 in various whole cell extracts using SLC25A1 polyclonal antibody (Product # PA5-85163) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A1 in mouse tissue extract using SLC25A1 polyclonal antibody (Product # PA5-85163) using 50 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A1 was performed by separating 30 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLC25A1 Polyclonal Antibody (Product # PA5-85163) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A1 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLC25A1 Polyclonal Antibody (Product # PA5-85163) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SLC25A1 in HepG2 cells using SLC25A1 polyclonal antibody (Product # PA5-85163) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.