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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [1]
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- Product number
- PA5-22971 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: human placenta lysate.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Maternal High-Fat Diet Consumption and Chronic Hyperandrogenemia Are Associated With Placental Dysfunction in Female Rhesus Macaques.
Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1.
Kuo K, Roberts VHJ, Gaffney J, Takahashi DL, Morgan T, Lo JO, Stouffer RL, Frias AE
Endocrinology 2019 Aug 1;160(8):1937-1949
Endocrinology 2019 Aug 1;160(8):1937-1949
Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1.
Kavanagh Williamson M, Coombes N, Juszczak F, Athanasopoulos M, Khan MB, Eykyn TR, Srenathan U, Taams LS, Dias Zeidler J, Da Poian AT, Huthoff H
Viruses 2018 Mar 7;10(3)
Viruses 2018 Mar 7;10(3)
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Multiple GLUT proteins are expressed in activated human CD4+ T cells and upregulated in response to infection with HIV-1. ( a ) The abundance of GLUT1-14, CD69, and IFNgamma mRNA in activated relative to resting CD4+ T cells at 5, 24, and 48 h after activation from six donors as determined by qPCR: n.d. indicates not detected. The data are averages after normalization to three reference genes (18S, B2M, and EIF4A2), with error bars representing the standard deviation; ( b ) Representative Western blotting analysis showing the detection of GLUT proteins in equal numbers of resting (R) and activated human CD4+ T cells at 5, 24, and 48 h post stimulation with GLUT-specific antibodies. Blots for GAPDH and HSP90 are included as controls; ( c ) The relative abundance of GLUT1-14 mRNA in HIV-1 infected versus uninfected cells as determined by qPCR of samples from 5 donors; ( d ) Representative Western blotting analysis of the expression of GLUT1, 3, 4, and 6 of uninfected (DeltaEnv) and HIV-1 NL4.3 infected primary CD4+ T cells in the presence or absence of nevirapine (NVP). Equal numbers of infected and uninfected cells were used, and HSP90 is shown as the loading control; ( e ) Quantified data from Western blotting analysis shown in ( d ) from six different donors. Significance is indicated by * p
