PA5-22966

antibody from Invitrogen Antibodies

Targeting: SLC2A9 Glut9, GLUTX, URATv1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22966

Provider

Invitrogen Antibodies

Product name

GLUT9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Cross-reactivity with human samples is very low. Suggested positive control: human kidney membrane protein.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hereditary hemochromatosis disrupts uric acid homeostasis and causes hyperuricemia via altered expression/activity of xanthine oxidase and ABCG2.

Ristic B, Sivaprakasam S, Narayanan M, Ganapathy V
The Biochemical journal 2020 Apr 30;477(8):1499-1513

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Expression of Glut9 and MRP4 in wild type and Hfe -/- mouse liver and intestinal tract. ( A ) Quantitative PCR analysis of Glut9 mRNA in liver and intestinal segments of wild-type mouse. Data show mean values +- SEM relative to liver, which is taken as 1. * P < 0.05; *** P < 0.001. ( B ) Western blot for Glut9 protein levels in liver and intestinal segments of the wild-type mouse. Western blot band intensities were estimated using ImageJ software and the band intensities were normalized to the respective beta-tubulin band intensities. Please see Supplementary Figures S5 and S10 for full blot images. ( C ) Quantitative PCR of Glut9 mRNA in liver, jejunum, ileum, and colon of Hfe -/- mice and wild-type mice. Data show mean values of three mice per group +- SEM relative to control mice. ( D ) Western blot for Glut9 protein levels in Hfe -/- mouse and wild-type mouse liver, jejunum, ileum, and colon. Western blot band intensities were estimated using ImageJ software and the band intensities were normalized to the respective beta-actin band intensities. Please see Supplementary Figures S8, S9, and S11 for full blot images. ( E ) Quantitative PCR analysis of Mrp4 mRNA in liver and intestinal segments of wild-type mouse. Data show mean values +- SEM relative to liver, which is taken as 1. * P < 0.05; *** P < 0.001. ( F ) Quantitative PCR analysis of Mrp4 mRNA in liver, jejunum, ileum, and colon of Hfe -/- mice and wild-type mice. Data show mean values of three mice per gro