BS-14387R

antibody from Invitrogen Antibodies

Targeting: DNAJC21 DNAJA5, GS3, JJJ1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

BS-14387R

Provider

Invitrogen Antibodies

Product name

DNAJC21 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: HT29 Cell lysates; Lane 2: mouse Cerebrum Cell lysates; Lane 3: Mouse kidney lysates; Lane 4: Mouse pancreas lysates; probed with DNAJC21 Polyclonal Antibody, unconjugated (bs-14387R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded mouse placenta tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DNAJC21 Polyclonal Antibody, Unconjugated (bs-14387R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.