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- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 15275-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15275-1-AP, RRID:AB_2256991
- Product name
- VAPA antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated VAPA antibody (Cat. #15275-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.
Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.
Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.
The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Upregulation of Rubicon promotes autosis during myocardial ischemia/reperfusion injury.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
A trap mutant reveals the physiological client spectrum of TRC40.
A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.
Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.
Gorkhali R, Tian L, Dong B, Bagchi P, Deng X, Pawar S, Duong D, Fang N, Seyfried N, Yang J
Scientific reports 2021 Oct 18;11(1):20576
Scientific reports 2021 Oct 18;11(1):20576
Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Culver JA, Mariappan M
The Journal of cell biology 2021 May 3;220(5)
The Journal of cell biology 2021 May 3;220(5)
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
Spits M, Heesterbeek IT, Voortman LM, Akkermans JJ, Wijdeven RH, Cabukusta B, Neefjes J
EMBO reports 2021 Mar 3;22(3):e50815
EMBO reports 2021 Mar 3;22(3):e50815
Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.
Nthiga TM, Shrestha BK, Bruun JA, Larsen KB, Lamark T, Johansen T
The Journal of cell biology 2021 Jun 7;220(6)
The Journal of cell biology 2021 Jun 7;220(6)
Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.
Liu N, Zhao H, Zhao YG, Hu J, Zhang H
The Journal of cell biology 2021 Jul 5;220(7)
The Journal of cell biology 2021 Jul 5;220(7)
The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway.
Cremer T, Jongsma MLM, Trulsson F, Vertegaal ACO, Neefjes J, Berlin I
Cell reports 2021 Jan 19;34(3):108659
Cell reports 2021 Jan 19;34(3):108659
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610
Scientific reports 2020 May 6;10(1):7610
Upregulation of Rubicon promotes autosis during myocardial ischemia/reperfusion injury.
Nah J, Zhai P, Huang CY, Fernández ÁF, Mareedu S, Levine B, Sadoshima J
The Journal of clinical investigation 2020 Jun 1;130(6):2978-2991
The Journal of clinical investigation 2020 Jun 1;130(6):2978-2991
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
Nthiga TM, Kumar Shrestha B, Sjøttem E, Bruun JA, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO journal 2020 Aug 3;39(15):e103649
The EMBO journal 2020 Aug 3;39(15):e103649
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science 2019 Jul 1;132(13)
Journal of cell science 2019 Jul 1;132(13)
A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.
Forrester A, De Leonibus C, Grumati P, Fasana E, Piemontese M, Staiano L, Fregno I, Raimondi A, Marazza A, Bruno G, Iavazzo M, Intartaglia D, Seczynska M, van Anken E, Conte I, De Matteis MA, Dikic I, Molinari M, Settembre C
The EMBO journal 2019 Jan 15;38(2)
The EMBO journal 2019 Jan 15;38(2)
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Freyre CAC, Rauher PC, Ejsing CS, Klemm RW
Molecular cell 2019 Dec 5;76(5):811-825.e14
Molecular cell 2019 Dec 5;76(5):811-825.e14
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
Zhao YG, Liu N, Miao G, Chen Y, Zhao H, Zhang H
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4
The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.
Zhao YG, Chen Y, Miao G, Zhao H, Qu W, Li D, Wang Z, Liu N, Li L, Chen S, Liu P, Feng D, Zhang H
Molecular cell 2017 Sep 21;67(6):974-989.e6
Molecular cell 2017 Sep 21;67(6):974-989.e6
Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.
Chong WM, Hsu SC, Kao WT, Lo CW, Lee KY, Shao JS, Chen YH, Chang J, Chen SS, Yu MJ
The Journal of biological chemistry 2016 Feb 19;291(8):3918-31
The Journal of biological chemistry 2016 Feb 19;291(8):3918-31
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human kidney tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP(VAPA antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VAPA antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VAPA. This antibody recognizes human,mouse,rat antigen. The VAPA antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 15275-1-AP(VAPA antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 15275-1-AP(VAPA antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
