15275-1-AP

antibody from Proteintech Group

Targeting: VAPA hVAP-33, VAP-A

Provider product page for 15275-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 15275-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15275-1-AP, RRID:AB_2256991
Product name: VAPA antibody
Antibody type: Polyclonal
Description: KD/KO validated VAPA antibody (Cat. #15275-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

VAPA protein structure - 15275-1-AP shown in red.

Submitted references Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation.

Accogli A, Shakya S, Yang T, Insinna C, Kim SY, Bell D, Butov KR, Severino M, Niceta M, Scala M, Lee HS, Yoo T, Stauffer J, Zhao H, Fiorillo C, Pedemonte M, Diana MC, Baldassari S, Zakharova V, Shcherbina A, Rodina Y, Fagerberg C, Roos LS, Wierzba J, Dobosz A, Gerard A, Potocki L, Rosenfeld JA, Lalani SR, Scott TM, Scott D, Azamian MS, Louie R, Moore HW, Champaigne NL, Hollingsworth G, Torella A, Nigro V, Ploski R, Salpietro V, Zara F, Pizzi S, Chillemi G, Ognibene M, Cooney E, Do J, Linnemann A, Larsen MJ, Specht S, Walters KJ, Choi HJ, Choi M, Tartaglia M, Youkharibache P, Chae JH, Capra V, Park SG, Westlake CJ
Nature communications 2024 Jan 8;15(1):365

VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity.

Bapat O, Purimetla T, Kruessel S, Shah M, Fan R, Thum C, Rupprecht F, Langer JD, Rangaraju V
Nature communications 2024 Jan 4;15(1):205

USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy.

Zhang M, Wang Z, Zhao Q, Yang Q, Bai J, Yang C, Zhang ZR, Liu Y
Autophagy 2024 Aug;20(8):1780-1797

RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress.

Cremer T, Voortman LM, Bos E, Jongsma ML, Ter Haar LR, Akkermans JJ, Talavera Ormeño CM, Wijdeven RH, de Vries J, Kim RQ, Janssen GM, van Veelen PA, Koning RI, Neefjes J, Berlin I
The EMBO journal 2023 Sep 18;42(18):e111252

CCDC127 regulates lipid droplet homeostasis by enhancing mitochondria-ER contacts.

Xia Y, Zhang Y, Sun Y, He L
Biochemical and biophysical research communications 2023 Nov 26;683:149116

Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.

Oh-Hashi K, Nakamura H, Ogawa H, Hirata Y, Sakurai K
International journal of molecular sciences 2023 Mar 17;24(6)

An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site.

Acevedo-Sánchez Y, Woida PJ, Kraemer S, Lamason RL
bioRxiv : the preprint server for biology 2023 Jun 6;

The CH24H metabolite, 24HC, blocks viral entry by disrupting intracellular cholesterol homeostasis.

Yuan Y, Fang A, Wang Z, Wang Z, Sui B, Zhu Y, Zhang Y, Wang C, Zhang R, Zhou M, Chen H, Fu ZF, Zhao L
Redox biology 2023 Aug;64:102769

Profiling of purified autophagic vesicle degradome in the maturing and aging brain.

Kallergi E, Siva Sankar D, Matera A, Kolaxi A, Paolicelli RC, Dengjel J, Nikoletopoulou V
Neuron 2023 Aug 2;111(15):2329-2347.e7

Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.

Zheng Q, Chen Y, Chen D, Zhao H, Feng Y, Meng Q, Zhao Y, Zhang H
Cell 2022 Oct 27;185(22):4082-4098.e22

Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis.

Kotani N, Nakano T, Kuwahara R
The Journal of biological chemistry 2022 Nov;298(11):102500

Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.

Gorkhali R, Tian L, Dong B, Bagchi P, Deng X, Pawar S, Duong D, Fang N, Seyfried N, Yang J
Scientific reports 2021 Oct 18;11(1):20576

Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.

Culver JA, Mariappan M
The Journal of cell biology 2021 May 3;220(5)

Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.

Spits M, Heesterbeek IT, Voortman LM, Akkermans JJ, Wijdeven RH, Cabukusta B, Neefjes J
EMBO reports 2021 Mar 3;22(3):e50815

Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

Nthiga TM, Shrestha BK, Bruun JA, Larsen KB, Lamark T, Johansen T
The Journal of cell biology 2021 Jun 7;220(6)

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Liu N, Zhao H, Zhao YG, Hu J, Zhang H
The Journal of cell biology 2021 Jul 5;220(7)

The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway.

Cremer T, Jongsma MLM, Trulsson F, Vertegaal ACO, Neefjes J, Berlin I
Cell reports 2021 Jan 19;34(3):108659

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610

Upregulation of Rubicon promotes autosis during myocardial ischemia/reperfusion injury.

Nah J, Zhai P, Huang CY, Fernández ÁF, Mareedu S, Levine B, Sadoshima J
The Journal of clinical investigation 2020 Jun 1;130(6):2978-2991

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Nthiga TM, Kumar Shrestha B, Sjøttem E, Bruun JA, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO journal 2020 Aug 3;39(15):e103649

A trap mutant reveals the physiological client spectrum of TRC40.

Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science 2019 Jul 1;132(13)

A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.

Forrester A, De Leonibus C, Grumati P, Fasana E, Piemontese M, Staiano L, Fregno I, Raimondi A, Marazza A, Bruno G, Iavazzo M, Intartaglia D, Seczynska M, van Anken E, Conte I, De Matteis MA, Dikic I, Molinari M, Settembre C
The EMBO journal 2019 Jan 15;38(2)

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Freyre CAC, Rauher PC, Ejsing CS, Klemm RW
Molecular cell 2019 Dec 5;76(5):811-825.e14

The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.

Zhao YG, Liu N, Miao G, Chen Y, Zhao H, Zhang H
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4

The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.

Zhao YG, Chen Y, Miao G, Zhao H, Qu W, Li D, Wang Z, Liu N, Li L, Chen S, Liu P, Feng D, Zhang H
Molecular cell 2017 Sep 21;67(6):974-989.e6

Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.

Chong WM, Hsu SC, Kao WT, Lo CW, Lee KY, Shao JS, Chen YH, Chang J, Chen SS, Yu MJ
The Journal of biological chemistry 2016 Feb 19;291(8):3918-31

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human kidney tissue were subjected to SDS PAGE followed by western blot with 15275-1-AP(VAPA antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The VAPA antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VAPA. This antibody recognizes human,mouse,rat antigen. The VAPA antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.
Appendix: 15275-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15275-1-AP(VAPA antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15275-1-AP(VAPA antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue