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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- PA5-22188 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3, mouse liver, rat liver.
- Concentration
- 1 mg/mL
Submitted references Role of Vesicle-Associated Membrane Protein-Associated Proteins (VAP) A and VAPB in Nuclear Egress of the Alphaherpesvirus Pseudorabies Virus.
Dorsch AD, Hölper JE, Franzke K, Zaeck LM, Mettenleiter TC, Klupp BG
Viruses 2021 Jun 10;13(6)
Viruses 2021 Jun 10;13(6)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAPA was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a VAPA Polyclonal Antibody (Product # PA5-22188) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VAPA Polyclonal Antibody (Product # PA5-22188). Sample (30 µg of whole cell lysate). A: H1299. 12% SDS PAGE. VAPA Polyclonal Antibody (Product # PA5-22188) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAPA was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a VAPA Polyclonal Antibody (Product # PA5-22188) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VAPA Polyclonal Antibody (Product # PA5-22188). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 12% SDS PAGE. VAPA antibody. VAPA Polyclonal Antibody (Product # PA5-22188) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using VAPA (Product # PA5-22188) antibody at 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using VAPA (Product # PA5-22188) antibody at 1:500 dilution.
