Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- HPA009174 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA009174, RRID:AB_1080549
- Product name
- Anti-VAPA
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human VAPA, Gene description: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa, Alternative Gene Names: hVAP-33, VAP-A, Validated applications: WB, IHC, ICC, Uniprot ID: Q9P0L0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2
Dynamic interactome of the MHC I peptide loading complex in human dendritic cells
Dual control of formin-nucleated actin assembly by the chromatin and ER in mouse oocytes
Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum
Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution
A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse
Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum
Nalbach K, Schifferer M, Bhattacharya D, Ho-Xuan H, Tseng W, Williams L, Stolz A, Lichtenthaler S, Elazar Z, Behrends C
Nature Communications 2023;14(1)
Nature Communications 2023;14(1)
Dynamic interactome of the MHC I peptide loading complex in human dendritic cells
Barends M, Koller N, Schölz C, Durán V, Bošnjak B, Becker J, Döring M, Blees H, Förster R, Kalinke U, Tampé R
Proceedings of the National Academy of Sciences 2023;120(25)
Proceedings of the National Academy of Sciences 2023;120(25)
Li X, Gamuyao R, Wu M, Cho W, Kurtz N, King S, Petersen R, Stabley D, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm R, Robinson C, Carisey A, Tebo A, Chang C
2023
2023
Dual control of formin-nucleated actin assembly by the chromatin and ER in mouse oocytes
Wang H, Hu J, Yi K, Ma Z, Song X, Lee Y, Kalab P, Bershadsky A, Miao Y, Li R
Current Biology 2022;32(18):4013-4024.e6
Current Biology 2022;32(18):4013-4024.e6
Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum
Shimasaki K, Kumagai K, Sakai S, Yamaji T, Hanada K
International Journal of Molecular Sciences 2022;23(7):4025
International Journal of Molecular Sciences 2022;23(7):4025
Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution
Tamura N, Sakai S, Martorell L, Colomé R, Mizuike A, Goto A, Ortigoza-Escobar J, Hanada K
Journal of Biological Chemistry 2021;297(5):101338
Journal of Biological Chemistry 2021;297(5):101338
A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse
Bates E, Leca I, Phillips A, Hofer I, Landler L, Ushakova L, Cushion T, Dürnberger G, Stejskal K, Mechtler K, Keays D
PLOS Genetics 2020;16(11):e1009104
PLOS Genetics 2020;16(11):e1009104
Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT
Srivastava A, Murakami H, Tamura N, Enomoto Y, Shimasaki K, Kurosawa K, Hanada K
PLOS ONE 2020;15(12):e0243980
PLOS ONE 2020;15(12):e0243980
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum
Cianciola N, Chung S, Manor D, Carlin C, Banks L
Journal of Virology 2017;91(6)
Journal of Virology 2017;91(6)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VAPA antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol