HPA009174

antibody from Atlas Antibodies

Targeting: VAPA hVAP-33, VAP-A

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA009174

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA009174, RRID:AB_1080549

Product name

Anti-VAPA

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human VAPA, Gene description: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa, Alternative Gene Names: hVAP-33, VAP-A, Validated applications: WB, IHC, ICC, Uniprot ID: Q9P0L0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2

Nalbach K, Schifferer M, Bhattacharya D, Ho-Xuan H, Tseng W, Williams L, Stolz A, Lichtenthaler S, Elazar Z, Behrends C
Nature Communications 2023;14(1)

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Dynamic interactome of the MHC I peptide loading complex in human dendritic cells

Barends M, Koller N, Schölz C, Durán V, Bošnjak B, Becker J, Döring M, Blees H, Förster R, Kalinke U, Tampé R
Proceedings of the National Academy of Sciences 2023;120(25)

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Li X, Gamuyao R, Wu M, Cho W, Kurtz N, King S, Petersen R, Stabley D, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm R, Robinson C, Carisey A, Tebo A, Chang C
2023

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Dual control of formin-nucleated actin assembly by the chromatin and ER in mouse oocytes

Wang H, Hu J, Yi K, Ma Z, Song X, Lee Y, Kalab P, Bershadsky A, Miao Y, Li R
Current Biology 2022;32(18):4013-4024.e6

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Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum

Shimasaki K, Kumagai K, Sakai S, Yamaji T, Hanada K
International Journal of Molecular Sciences 2022;23(7):4025

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Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution

Tamura N, Sakai S, Martorell L, Colomé R, Mizuike A, Goto A, Ortigoza-Escobar J, Hanada K
Journal of Biological Chemistry 2021;297(5):101338

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A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse

Bates E, Leca I, Phillips A, Hofer I, Landler L, Ushakova L, Cushion T, Dürnberger G, Stejskal K, Mechtler K, Keays D
PLOS Genetics 2020;16(11):e1009104

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Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT

Srivastava A, Murakami H, Tamura N, Enomoto Y, Shimasaki K, Kurosawa K, Hanada K
PLOS ONE 2020;15(12):e0243980

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Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum

Cianciola N, Chung S, Manor D, Carlin C, Banks L
Journal of Virology 2017;91(6)

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VAPA antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Sample type

Human

Protocol

Protocol