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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- HPA040343 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA040343, RRID:AB_2676930
- Product name
- Anti-SPEF2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPEF2, Gene description: sperm flagellar 2, Alternative Gene Names: CT122, FLJ23577, KPL2, Validated applications: IHC, Uniprot ID: Q9C093, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Novel HYDIN variants associated with male infertility in two Chinese families
Sperm flagellar 2 (SPEF2) is essential for sperm flagellar assembly in humans
Bi-allelic variants in DNHD1 cause flagellar axoneme defects and asthenoteratozoospermia in humans and mice
Islet primary cilia motility controls insulin secretion.
Bi-allelic Loss-of-function Variants in CFAP58 Cause Flagellar Axoneme and Mitochondrial Sheath Defects and Asthenoteratozoospermia in Humans and Mice
Mutations in ARL2BP, a protein required for ciliary microtubule structure, cause syndromic male infertility in humans and mice
Bi-allelic Mutations in ARMC2 Lead to Severe Astheno-Teratozoospermia Due to Sperm Flagellum Malformations in Humans and Mice
Absence of CFAP69 Causes Male Infertility due to Multiple Morphological Abnormalities of the Flagella in Human and Mouse
Yu H, Shi X, Shao Z, Geng H, Guo S, Li K, Gu M, Xu C, Gao Y, Tan Q, Duan Z, Wu H, Hua R, Guo R, Wei Z, Zhou P, Cao Y, He X, Li L, Zhang X, Lv M
Frontiers in Endocrinology 2023;14
Frontiers in Endocrinology 2023;14
Sperm flagellar 2 (SPEF2) is essential for sperm flagellar assembly in humans
Li D, Yang X, Tu C, Wang W, Meng L, Lu G, Tan Y, Zhang Q, Du J
Asian Journal of Andrology 2022;24(4):359-366
Asian Journal of Andrology 2022;24(4):359-366
Bi-allelic variants in DNHD1 cause flagellar axoneme defects and asthenoteratozoospermia in humans and mice
Tan C, Meng L, Lv M, He X, Sha Y, Tang D, Tan Y, Hu T, He W, Tu C, Nie H, Zhang H, Du J, Lu G, Fan L, Cao Y, Lin G, Tan Y
The American Journal of Human Genetics 2022;109(1):157-171
The American Journal of Human Genetics 2022;109(1):157-171
Islet primary cilia motility controls insulin secretion.
Cho JH, Li ZA, Zhu L, Muegge BD, Roseman HF, Lee EY, Utterback T, Woodhams LG, Bayly PV, Hughes JW
Science advances 2022 Sep 23;8(38):eabq8486
Science advances 2022 Sep 23;8(38):eabq8486
Bi-allelic Loss-of-function Variants in CFAP58 Cause Flagellar Axoneme and Mitochondrial Sheath Defects and Asthenoteratozoospermia in Humans and Mice
He X, Liu C, Yang X, Lv M, Ni X, Li Q, Cheng H, Liu W, Tian S, Wu H, Gao Y, Yang C, Tan Q, Cong J, Tang D, Zhang J, Song B, Zhong Y, Li H, Zhi W, Mao X, Fu F, Ge L, Shen Q, Zhang M, Saiyin H, Jin L, Xu Y, Zhou P, Wei Z, Zhang F, Cao Y
The American Journal of Human Genetics 2020;107(3):514-526
The American Journal of Human Genetics 2020;107(3):514-526
Mutations in ARL2BP, a protein required for ciliary microtubule structure, cause syndromic male infertility in humans and mice
O'Bryan M, Moye A, Bedoni N, Cunningham J, Sanzhaeva U, Tucker E, Mathers P, Peter V, Quinodoz M, Paris L, Coutinho-Santos L, Camacho P, Purcell M, Winkelmann A, Foster J, Pugacheva E, Rivolta C, Ramamurthy V
PLOS Genetics 2019;15(8):e1008315
PLOS Genetics 2019;15(8):e1008315
Bi-allelic Mutations in ARMC2 Lead to Severe Astheno-Teratozoospermia Due to Sperm Flagellum Malformations in Humans and Mice
Coutton C, Martinez G, Kherraf Z, Amiri-Yekta A, Boguenet M, Saut A, He X, Zhang F, Cristou-Kent M, Escoffier J, Bidart M, Satre V, Conne B, Fourati Ben Mustapha S, Halouani L, Marrakchi O, Makni M, Latrous H, Kharouf M, Pernet-Gallay K, Bonhivers M, Hennebicq S, Rives N, Dulioust E, Touré A, Gourabi H, Cao Y, Zouari R, Hosseini S, Nef S, Thierry-Mieg N, Arnoult C, Ray P
The American Journal of Human Genetics 2019;104(2):331-340
The American Journal of Human Genetics 2019;104(2):331-340
Absence of CFAP69 Causes Male Infertility due to Multiple Morphological Abnormalities of the Flagella in Human and Mouse
Dong F, Amiri-Yekta A, Martinez G, Saut A, Tek J, Stouvenel L, Lorès P, Karaouzène T, Thierry-Mieg N, Satre V, Brouillet S, Daneshipour A, Hosseini S, Bonhivers M, Gourabi H, Dulioust E, Arnoult C, Touré A, Ray P, Zhao H, Coutton C
The American Journal of Human Genetics 2018;102(4):636-648
The American Journal of Human Genetics 2018;102(4):636-648
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human fallopian tube and skeletal muscle tissues using HPA040343 antibody. Corresponding SPEF2 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
