14453-1-AP

antibody from Proteintech Group

Targeting: CD276 B7-H3, B7H3, B7RP-2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

14453-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14453-1-AP, RRID:AB_2073577

Product name

B7-H3 antibody

Antibody type

Polyclonal

Description

KD/KO validated B7-H3 antibody (Cat. #14453-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Engineered CAR-T-Derived Exosomes Co-Delivering miR-145 and Cytotoxic Proteins for Targeted Solid Tumour Therapy.

Yang R, Wang H, Wang G, Yuan G, Wei Z, An J, Hu D, Wen W, Gao S, Wan Y, Liang G
Journal of extracellular vesicles 2026 Mar;15(3):e70245

---

Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3.

Lu Y, Wang K, Peng Y, Chen M, Zhong L, Huang L, Cheng FU, Sheng X, Yang X, Ouyang M, Calin GA, He Z
Oncology research 2025;33(1):103-121

---

EPB41L3 as a prognostic biomarker suppressing cellular malignancy and correlating with the immune microenvironment in glioma.

Liu X, Liu Y, Du K, Wang C, Zhang Y, Li L, Wang D, Chen L, Nov P, Li J, Zeng R
Biochemical and biophysical research communications 2025 Sep 8;778:152305

---

B7-H3-mediated deubiquitination stabilizing CYP1B1 expression promotes chemotherapy resistance in colorectal cancer.

Liu H, Li G, Shen C, Qi X, Liu Y, Hua D, Mao Y, Zhang T
Biochimica et biophysica acta. Molecular basis of disease 2025 Jun;1871(5):167771

---

Pan-cancer analysis reveals SMARCAL1 expression is associated with immune cell infiltration and poor prognosis in various cancers.

Zhao WJ, Wang ML, Zhao YF, Zhao WP, Huang QH, Lu ZW, Jia F, Shi JJ, Liu BS, Han WH, Lu HW, Zhang BC, Wang ZX
Scientific reports 2025 Feb 24;15(1):6591

---

B7-H3 suppresses CD8(+) T cell immunologic function through reprogramming glycolytic metabolism.

Wu Y, Han W, Tang X, Liu J, Guo Z, Li Z, Cai C, Que L
Journal of Cancer 2024;15(9):2505-2517

---

Neoadjuvant immunochemotherapy improves clinical outcomes of patients with esophageal cancer by mediating anti-tumor immunity of CD8+ T (Tc1) and CD16+ NK cells.

He Y, Yang D, Lin X, Zhang J, Cheng R, Cao L, Yang L, Zhang M, Shi X, Jin X, Sun H, Sun H, Zang J, Li Y, Ma J, Nie H
Frontiers in immunology 2024;15:1412693

---

B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway.

Zhao A, Zhu X, Wu H, Wang J, Zhang M, Xiang J, Xia S, Shi T, Xi Q
Tissue & cell 2024 Oct;90:102518

---

PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability.

Yu J, Xiang Y, Gao Y, Chang S, Kong R, Lv X, Yu J, Jin Y, Li C, Ma Y, Wang Z, Zhou J, Yuan H, Shang S, Hua F, Zhang X, Cui B, Li P
Acta pharmaceutica Sinica. B 2024 Oct;14(10):4378-4395

---

Integrin β5, a noninvasive diagnostic biomarker, is associated with unfavorable prognosis and immunotherapy efficacy in gastric cancer.

Cheng Y, Lin X, Xu H, Xu M, Xie W, Zeng J, Cui B, Cheng Z, Zhao J, Sun Y
BMC gastroenterology 2024 Oct 11;24(1):362

---

N6-methyladenosine modification of B7-H3 mRNA promotes the development and progression of colorectal cancer.

Chen R, Su F, Zhang T, Wu D, Yang J, Guan Q, Chai C
iScience 2024 Feb 16;27(2):108956

---

COL10A1 promotes tumorigenesis by modulating CD276 in pancreatic adenocarcinoma.

Xu Q, Zheng J, Su Z, Chen B, Gu S
BMC gastroenterology 2023 Nov 16;23(1):397

---

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression.

Zhang Q, Cao W, Yang C, Hong L, Geng S, Han H, Zhong C
The Journal of nutritional biochemistry 2023 Feb;112:109226

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B7-H3 confers stemness characteristics to gastric cancer cells by promoting glutathione metabolism through AKT/pAKT/Nrf2 pathway.

Xia L, Chen Y, Li J, Wang J, Shen K, Zhao A, Jin H, Zhang G, Xi Q, Xia S, Shi T, Li R
Chinese medical journal 2023 Aug 20;136(16):1977-1989

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PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

Zhang H, Wang Y, Zhao Y, Liu T, Wang Z, Zhang N, Dai Z, Wu W, Cao H, Feng S, Zhang L, Cheng Q, Liu Z
CNS neuroscience & therapeutics 2022 Nov;28(11):1748-1766

---

B7-H3 targeted antibody-based immunotherapy of malignant diseases.

Michelakos T, Kontos F, Barakat O, Maggs L, Schwab JH, Ferrone CR, Ferrone S
Expert opinion on biological therapy 2021 May;21(5):587-602

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Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.

Li QT, Qiu MJ, Yang SL, Fang X, He XX, Wang MM, Li YN, Xiong ZF, Huang S
Journal of oncology 2020;2020:9327512

---

The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.

Li Z, Liu J, Que L, Tang X
Journal of Cancer 2019;10(23):5770-5784

---

Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.

Tang L, He S, Zhu Y, Feng B, Su Z, Liu B, Xu F, Wang X, Liu H, Li C, Zhao J, Zheng X, Li C, Sun C, Lu C, Zheng G
Journal of cellular physiology 2019 Apr;234(4):3661-3674

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Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse.

Li K, Wei X, Zhang G, Li M, Zhang X, Zhou C, Hou J, Yuan H
BMC urology 2017 Apr 4;17(1):23

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B7-H3 in combination with regulatory T cell is associated with tumor progression in primary human non-small cell lung cancer.

Jin Y, Zhang P, Li J, Zhao J, Liu C, Yang F, Yang D, Gao A, Lin W, Ma X, Sun Y
International journal of clinical and experimental pathology 2015;8(11):13987-95

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Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.

Li M, Zhang G, Zhang X, Lv G, Wei X, Yuan H, Hou J
Medical oncology (Northwood, London, England) 2014 Dec;31(12):349

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 14453-1-AP(CD276 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human medulloblastoma using 14453-1-AP(CD276 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human medulloblastoma using 14453-1-AP(CD276 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CD276 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CD276. This antibody recognizes human,mouse,rat antigen. The CD276 antibody has been validated for the following applications: ELISA, WB, IHC, FC analysis.

Appendix

14453-1-AP.pdf

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