PAB29313

antibody from Abnova Corporation

Targeting: CD276 B7-H3, B7H3, B7RP-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29313

Provider

Abnova Corporation

Product name

CD276 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human CD276.

Antigen sequence

EVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLN
LIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDL
LAQGNASLRLQRVRVADEGSFTCFVSIRDFG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CD276 polyclonal antibody (Cat# PAB29313) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence staining of A-431 cells with CD276 polyclonal antibody (Cat# PAB29313) under 1-4 ug/mL working concentration.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate tissue with CD276 polyclonal antibody (Cat# PAB29313) at 1:50 - 1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)