PA5-51098

antibody from Invitrogen Antibodies

Targeting: CD276 B7-H3, B7H3, B7RP-2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51098

Provider

Invitrogen Antibodies

Product name

B7-H3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

B7-H3 / CD276 Polyclonal Antibody detects endogenous levels of B7-H3 / CD276 protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells.

Knox T, Sahakian E, Banik D, Hadley M, Palmer E, Noonepalle S, Kim J, Powers J, Gracia-Hernandez M, Oliveira V, Cheng F, Chen J, Barinka C, Pinilla-Ibarz J, Lee NH, Kozikowski A, Villagra A
Scientific reports 2019 Apr 16;9(1):6136

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD276 in 293 cells. Samples were probed using CD276 Polyclonal Antibody (Product # PA5-51098).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 The up-regulation of PD-L1 in anti-PD-1 treated mice is mediated by IFNgamma. ( A ) C57BL/6 mice were subcutaneously injected with 1 x 10 6 SM1 murine melanoma tumor cells. Mice were treated with 15 mg/kg anti-PD-1 or a vehicle control for 21 days. Tumor nodules were isolated to evaluate the expression of IFNgamma by qRT-PCR ( B ), and PD-L1, PD-L2, B7-H3, B7-H4, OX40L, and GAPDH by immunoblot ( C ). SM1 melanoma cells were treated in vitro with NextA or vehicle and then co-cultured with CD3/CD28 activated splenocytes in the presence or absence of IFNgamma blocking antibody at 1:1000 and 1:100 dilutions. Then, the expression of PD-L1 was analyzed by qRT-PCR ( D ), and the expression of IFNgamma by ELISA ( E ).