Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-51098 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B7-H3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- B7-H3 / CD276 Polyclonal Antibody detects endogenous levels of B7-H3 / CD276 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells.
Knox T, Sahakian E, Banik D, Hadley M, Palmer E, Noonepalle S, Kim J, Powers J, Gracia-Hernandez M, Oliveira V, Cheng F, Chen J, Barinka C, Pinilla-Ibarz J, Lee NH, Kozikowski A, Villagra A
Scientific reports 2019 Apr 16;9(1):6136
Scientific reports 2019 Apr 16;9(1):6136
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD276 in 293 cells. Samples were probed using CD276 Polyclonal Antibody (Product # PA5-51098).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 The up-regulation of PD-L1 in anti-PD-1 treated mice is mediated by IFNgamma. ( A ) C57BL/6 mice were subcutaneously injected with 1 x 10 6 SM1 murine melanoma tumor cells. Mice were treated with 15 mg/kg anti-PD-1 or a vehicle control for 21 days. Tumor nodules were isolated to evaluate the expression of IFNgamma by qRT-PCR ( B ), and PD-L1, PD-L2, B7-H3, B7-H4, OX40L, and GAPDH by immunoblot ( C ). SM1 melanoma cells were treated in vitro with NextA or vehicle and then co-cultured with CD3/CD28 activated splenocytes in the presence or absence of IFNgamma blocking antibody at 1:1000 and 1:100 dilutions. Then, the expression of PD-L1 was analyzed by qRT-PCR ( D ), and the expression of IFNgamma by ELISA ( E ).