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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- 16-5937-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD276 (B7-H3) Monoclonal Antibody (MIH35), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2). Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis. Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- MIH35
- Vial size
- 500 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.
Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2) is a counter-receptor for B7-H3 and enhances T cell responses.
B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production.
Lu H, Shi T, Wang M, Li X, Gu Y, Zhang X, Zhang G, Chen W
Oncoimmunology 2020;9(1):1748991
Oncoimmunology 2020;9(1):1748991
Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2) is a counter-receptor for B7-H3 and enhances T cell responses.
Hashiguchi M, Kobori H, Ritprajak P, Kamimura Y, Kozono H, Azuma M
Proceedings of the National Academy of Sciences of the United States of America 2008 Jul 29;105(30):10495-500
Proceedings of the National Academy of Sciences of the United States of America 2008 Jul 29;105(30):10495-500
B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production.
Chapoval AI, Ni J, Lau JS, Wilcox RA, Flies DB, Liu D, Dong H, Sica GL, Zhu G, Tamada K, Chen L
Nature immunology 2001 Mar;2(3):269-74
Nature immunology 2001 Mar;2(3):269-74
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of B7-H3-transfected cells with 0.25 µg of Rat IgG2a Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.5 µg of Anti-Human/Mouse CD276 (B7-H3) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG PE (Product # 12-4822).Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
