Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- LS-B15844 - Provider product page
- Provider
- LSBio
- Product name
- PathPlus™ CD276 / B7-H3 Antibody (aa47-219) LS-B15844
- Antibody type
- Polyclonal
- Description
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of recombinant CD276 / B7-H3.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Knockout Varification: Lane 1: Wild-type 293T cell lysate; Lane 2: CD276 knockout 293T cell lysate; Predicted MW: 57,53,33kd Observed MW: 100kd Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody