NBP2-75195

antibody from Novus Biologicals

Targeting: CD276 B7-H3, B7H3, B7RP-2

ELISA (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP2-75195

Provider

Novus Biologicals

Product name

Rabbit Monoclonal B7-H3/CD276 Antibody

Antibody type

Monoclonal

Description

Protein A purified. This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: B7-H3/CD276 Antibody (MJ18) [NBP2-75195] - Binding curve of anti-B7-H3 antibody MJ18 (NBP2-75195) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-75195. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: B7-H3/CD276 Antibody (MJ18) [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody