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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-36279 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD276 (B7-H3) Monoclonal Antibody (2H5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive controls that may be used include: HEK293 Cell Lysate, HEK293 Cell Slide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279) at (A) 0.25 (B) 0.5 and (C) 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279), CD276 (B7-H3) Monoclonal Antibody (2A7) (Product # MA5-36280), and CD276 (B7-H3) Monoclonal Antibody (4H3) (Product # MA5-36281) at decreasing concentrations.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279) and control mouse IgG antibody (left corner box) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279) at 5 µg/mL. Green: B7-H3 Antibody [2H5] (RF16091) Blue: DAPI staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of B7-H3 in human colon carcinoma tissue using CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279) and control mouse IgG (corner box) at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279) at 1 µg/mL. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
