Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-36280 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD276 (B7-H3) Monoclonal Antibody (2A7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive controls that may be used include: HEK293 Cell Lysate, HEK293 Cell Slide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A7
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with CD276 (B7-H3) Monoclonal Antibody (2H5) (Product # MA5-36279), CD276 (B7-H3) Monoclonal Antibody (2A7) (Product # MA5-36280), and CD276 (B7-H3) Monoclonal Antibody (4H3) (Product # MA5-36281) at decreasing concentrations.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2A7) (Product # MA5-36280) and control mouse IgG antibody (left corner box) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2A7) (Product # MA5-36280) at 5 µg/mL. Green: B7-H3 Antibody [2A7] (RF16092) Blue: DAPI staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of B7-H3 in HEK293 cells using CD276 (B7-H3) Monoclonal Antibody (2A7) (Product # MA5-36280) at 1 µg/mL. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
