80381-MSM5-P0

antibody from Invitrogen Antibodies

Targeting: CD276 B7-H3, B7H3, B7RP-2

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

80381-MSM5-P0

Provider

Invitrogen Antibodies

Product name

CD276/B7-H3 Monoclonal Antibody (B7H3/4345)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

B7H3/4345

Vial size

20 µg

Concentration

200 µg/mL

Storage

4° C, do not freeze

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CD276/B7-H3 Mouse Monoclonal Antibody (B7H3/4345) at 2 µg/mL. HIER: Tris/EDTA, pH9.0, 45 min. 2 °: HRP-polymer, 30 min. DAB, 5 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CD276/B7-H3 Mouse Monoclonal Antibody (B7H3/4345) at 2 µg/mL. HIER: Tris/EDTA, pH9.0, 45 min. 2 °: HRP-polymer, 30 min. DAB, 5 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD276/B7-H3 Monospecific Mouse Monoclonal Antibody (B7H3/4345). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the diFerence (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.