Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- Ab094381 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD276 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human CD276 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.ApplicationWB: 0.01-3 µg/mLIHC: 5-30 µg/mLProtein FunctionMay participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 47-219
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD276 Antibody (Ab094381) - Western Blot All lanes: CD276 Antibody (Ab094381) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 90 kDa Observed band size: 92 kDa Exposure time: 71.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD276 Antibody (Ab094381) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 μg/mL CD276 Antibody (Ab094381) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD276 Antibody (Ab094381) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 μg/mL CD276 Antibody (Ab094381) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD276 Antibody (Ab094381) - Flow Cytometry CHO-K1 transfected with human CD276 (Stable transfection) was stained with CD276 Antibody (Ab094381) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by Cy5-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.