Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-15693 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B7-H3 Monoclonal Antibody (6A1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15693 targets CD276 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-15693 immunogen is purified recombinant fragment of human CD276 expressed in E. Coli. MA5-15693 detects CD276 which has a predicted molecular weight of approximately 57kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6A1
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Humanized Affinity-matured Monoclonal Antibody 8H9 Has Potent Antitumor Activity and Binds to FG Loop of Tumor Antigen B7-H3.
Ahmed M, Cheng M, Zhao Q, Goldgur Y, Cheal SM, Guo HF, Larson SM, Cheung NK
The Journal of biological chemistry 2015 Dec 11;290(50):30018-29
The Journal of biological chemistry 2015 Dec 11;290(50):30018-29
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD276/B7H3 using CD276/B7H3 monoclonal antibody (Product # MA5-15693) in HEK293 (1) and CD276 (AA: 30-130) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 differentiated to Dendritic cells (Lane 2), LNCaP (Lane 3), U-2 OS (Lane 4), PC-3 (Lane 5), HeLa (Lane 6), MCF7 (Lane 7), Jurkat (Lane 8) and Raji (Lane 9). The blot was probed with Anti-B7-H3 Monoclonal Antibody (6A1) (Product # MA5-15693, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 100 kDa band corresponding to B7-H3 was detected in THP-1 differentiated to Dendritic cells, LNCaP, U-2 OS, PC-3, HeLa and MCF7 and not in Jurkat and Raji which are reported negative for B7-H3 expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276/B7H3 monoclonal antibody (Product # MA5-15693) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PC-3 cells using CD276/B7H3 monoclonal antibody (Product # MA5-15693) (green) and negative control (purple).