AF7006

antibody from R&D Systems

Targeting: EBF2 COE2, FLJ11500

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7006

Provider

R&D Systems

Product name

Human/Mouse EBF-2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse EBF-2 in direct ELISAs. Detects mouse and human EBF-2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) EBF-1 and rmEBF-3 is observed.

Reactivity

Human, Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

O08792


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting.

Elattar S, Dimri M, Satyanarayana A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Sep;32(9):4727-4743

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A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome.

Su S, Guntur AR, Nguyen DC, Fakory SS, Doucette CC, Leech C, Lotana H, Kelley M, Kohli J, Martino J, Sims-Lucas S, Liaw L, Vary C, Rosen CJ, Brown AC
Cell reports 2018 Dec 11;25(11):3215-3228.e9

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Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning.

Patil M, Sharma BK, Elattar S, Chang J, Kapil S, Yuan J, Satyanarayana A
Diabetes 2017 Jun;66(6):1611-1625

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A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells.

Velasco S, Ibrahim MM, Kakumanu A, Garipler G, Aydin B, Al-Sayegh MA, Hirsekorn A, Abdul-Rahman F, Satija R, Ohler U, Mahony S, Mazzoni EO
Cell stem cell 2017 Feb 2;20(2):205-217.e8

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A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.

Zhao XY, Li S, Wang GX, Yu Q, Lin JD
Molecular cell 2014 Aug 7;55(3):372-82

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EBF-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

EBF-2 in Mouse Embryo. EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.