LS-B345

antibody from LSBio

Targeting: AP3D1 ADTD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B345

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B345, RRID:AB_2056633

Product name

IHC-plus™ AP3D1 / Adaptin Delta Antibody (aa675-700) LS-B345

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Chicken/Avian

Host

Goat

Isotype

IgG

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-AP3D1 Antibody - Western Blot. Western blot of Affinity Purified anti-AP3D1 antibody shows detection of a 130-kD band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.