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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB9965 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9965, RRID:AB_1672113
- Product name
- AP3D1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of AP3D1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of the adaptor-related protein complex, AP-3.
Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet.
Simpson F, Peden AA, Christopoulou L, Robinson MS
The Journal of cell biology 1997 May 19;137(4):835-45
The Journal of cell biology 1997 May 19;137(4):835-45
Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet.
Ooi CE, Moreira JE, Dell'Angelica EC, Poy G, Wassarman DA, Bonifacino JS
The EMBO journal 1997 Aug 1;16(15):4508-18
The EMBO journal 1997 Aug 1;16(15):4508-18
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using AP3D1 polyclonal antibody (Cat # PAB9965) shows detection of a 130 KDa band corresponding to Human AP3D1 in a HeLa whole cell lysate.The lower molecular weight band most likely represents non-specificbinding.Approximately 20 ug of lysate was run ona SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP3D1 polyclonal antibody.Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-GoatIgG for 1 hour at room temperature.A chemilumi-nescence system was used for signal detection using a 30-sec exposure time.
