Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-101-290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AP3D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Concentration
- 1.6 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot using Rocklands Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 30-sec exposure time.