A300-538A

antibody from Invitrogen Antibodies

Targeting: PDS5B APRIN, AS3, CG008, FLJ23236, KIAA0979

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-538A

Provider

Invitrogen Antibodies

Product name

Pds5B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pds5b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pds5b antibody A300-538A (lot A300-538A-2) used for IP at 3 µg per reaction. Pds5b was also immunoprecipitated by rabbit anti-Pds5b antibody A300-537A. For blotting immunoprecipitated Pds5b, A300-538A was used at 1 µg/ml. Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Pds5b by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pds5b antibody A300-538A (lot A300-538A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pds5B by immunohistochemistry. Sample: FFPE section of human lung small cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B (Cat. No. A300-538A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB.