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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-68303 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with Mouse and Rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references A Missense Mutation in RAB28 in a Family with Cone-Rod Dystrophy and Postaxial Polydactyly Prevents Localization of RAB28 to the Primary Cilium.
Jespersgaard C, Hey AB, Ilginis T, Hjortshøj TD, Fang M, Bertelsen M, Bech N, Jensen H, Larsen LJ, Tümer Z, Rosenberg T, Brøndum-Nielsen K, Møller LB, Grønskov K
Investigative ophthalmology & visual science 2020 Feb 7;61(2):29
Investigative ophthalmology & visual science 2020 Feb 7;61(2):29
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB28 in COLO205 whole cell lysates using a RAB28 Polyclonal Antibody (Product # PA5-68303).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. (A) Serum reduction leads to increased expression of RAB28 . Expression profiles of RAB28 mRNA were normalized to the amount of endogenous GADPH mRNA. A significant increase in the level of RAB28 was observed for all samples under reduced-serum conditions compared to standard growth conditions ( ** P = 0.001815 for control; * P = 0.007181 for IV-1; ** P = 0.003329 for IV-3). Three independent experiments were performed with similar results. Statistical analysis was performed using t -tests. (B) Ciliary localization of RAB28 is significantly reduced in patient cell lines. IFM analysis of control and patient cells grown under reduced-serum conditions. Cilia were labeled with anti-acetylated alfa-tubulin (AC-TUB) antibody (red). RAB28 protein (green) can be observed in the cilia in the two control cells (white arrows), whereas none of the three displayed patient cells (IV-1) shows ciliary localization of RAB28. Nuclei were visualized with DAPI staining (blue). The ciliary localization of RAB28 was significantly reduced in both patient cell lines compared to control (see Table 2 ). Scale bar, 10 um. (C) siRNA-mediated knock down of RAB28 leads to a reduction of ciliary RAB28. Normalized expression profile of RAB28 mRNA expression in control cells treated with siRNA is compared with RAB28 (siRAB28) and a negative control (siScramble) grown under serum reduced conditions. Expression profiles of RAB28 were normalized to the amount of endogenous GAPDH mRNA. The expression l
