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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-30784 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BNIP3L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L (4)..
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BNIP3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide using a BNIP3L polyclonal antibody (Product # PA1-30784) at a dilution of 1µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-BNIP3L Polyclonal Antibody (Product # PA1-30784) and 36, 40 kDa bands corresponding to BNIP3L were observed across all the cell lines and tissue tested. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HEL 92.1.7 (Lane 2), K-562 (Lane 3), HeLa (Lane 4), SK-BR-3 (Lane 5) and tissue extracts of Mouse Spleen (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
