12986-1-AP

antibody from Proteintech Group

Targeting: BNIP3L BNIP3a, Nix

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

12986-1-AP

Provider

Proteintech Group

Product name

BNIP3L antibody

Antibody type

Polyclonal

Description

BNIP3L antibody (Cat. #12986-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Rg1-R1 attenuates cardiac ischemia/reperfusion-induced endothelial cell injury through activating the ULK1/PGAM5-FUNDC1-mitophagy pathway.

Song X, Deng J, Wang D, Zheng Z, Li C, Li J
Journal of ginseng research 2026 Mar;50(2):100973

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EGR2 affects mixed stroke repair through BNIP3L-mediated microglial mitophagy.

Zhang X, Zhang B, Shi C, Kampan N, Treebupachatsakul W, Pantan R, Narakornsak S, Phatsara M
IBRO neuroscience reports 2026 Jun;20:577-586

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Autophagy regulates PVALB (parvalbumin) interneuron excitability and memory.

Chalatsi T, Wosnitzka E, Kolaxi A, Fernandez LMJ, Scholler J, Batti L, Restivo L, Knott G, Lüthi A, Mameli M, Nikoletopoulou V
Autophagy 2026 Feb;22(2):351-370

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MeCP2 dysregulation inhibits mitophagy and impairs neural development in cortical organoids.

Zhou J, Liu Y, Jing X, Peng H, Li F, Cao L, Li W, Li R, Zhang J, Wang X, Lian J, Tong D, Huang C
Journal of advanced research 2026 Apr;82:429-448

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Diosmetin alleviates hyperuricemic nephropathy by activating the BNIP3/Nrf2/GPX4 pathway to inhibit ferroptosis of renal tubular epithelial cells induced by monosodium urate crystals.

Hu C, Dong C, Yang H, Yu Z, Li Y, Qiu Y, Mei Z, Xu L
The Journal of nutritional biochemistry 2026 Apr;150:110241

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Pestiviruses utilize pyrimidine metabolism to regulate mitophagy for viral replication.

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Autophagy 2026 Apr 20;:1-22

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Decidual protein induced by progesterone enhances HIF-1α stability to promote mitophagy and glycolysis.

Tian B, Shen J, Chu X, Zhou Y, Li H, Wang L, Guo M, Dou J
The FEBS journal 2026 Apr 1;

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Salidroside Attenuates Cerebral Ischemia-Reperfusion Injury via ERβ/BNIP3-Mediated Mitochondrial Autophagy Activation in a Rat Model.

Rong X, Lu P, Li Y, Wang H, Yue Y, Wang H, Ji Z, Jiang L
Neurochemical research 2025 Sep 11;50(5):297

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Pharmacological induction of acetyl-CoA carboxylase 1 autophagic degradation attenuates lipid accumulation and cholangiocarcinoma progression.

Pan Y, Zhang N, Fu X, Wang X, Ma Y, Chen Q, Zhou Y, Liu H, Zhu Y, Xu L, Wang Q, Chen D, Wang Z, Wang L
Journal of experimental & clinical cancer research : CR 2025 Nov 25;44(1):310

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Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline.

Zhang Y, Fan T
Antioxidants (Basel, Switzerland) 2025 Nov 19;14(11)

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Mitophagy-mtROS axis contributes to anti-tuberculosis-induced liver injury through activation of the cGAS-STING pathway in rat hepatocytes.

Chen W, Luo C, Zhou H, Liu Z, Huang J, Liu Y, You M, Yang G
International immunopharmacology 2025 Jul 28;160:114984

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PINK1 overexpression suppresses p38 MAPK/NF‑κB signaling to attenuate chondrocyte senescence in osteoarthritis.

Jie L, Zhang Y, Liu J, Fu H, Zhu Z, Huang Z, Shi X, Wang P, Yin S
International journal of molecular medicine 2025 Dec;56(6)

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Capsaicin attenuates sepsis‑associated encephalopathy by inhibiting neuroinflammation and apoptosis whilst activating mitophagy through the BNIP3/NIX pathway.

Zhang S, Luo N, Wu H, Chen J, Jiang Y, Xiao L, Liang H, Xue Q, Luo Y, Yu B, Liu Y, Liu Z
Molecular medicine reports 2025 Dec;32(6)

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PABPC1 SUMOylation enhances cell survival by promoting mitophagy through stabilizing U-rich mRNAs within stress granules.

Huang C, Huang J, Lu R, Yu AJ, Yu AS, Cao Y, Li L, Li J, Li H, Zhou Z, Zhang Y, Xu A, Chen R, Wang Y, Zhao X, Huang J, Fu Y, Xu M, Zhang H, Yu J
Nature communications 2025 Aug 7;16(1):7308

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Camellia japonica Flower Extract and the Active Constituent Hyperoside Repair DNA Damage Through FUNDC1-Mediated Mitophagy Pathway for Skin Anti-Aging.

Gao H, Shi J, Li G, Lai Z, Liu Y, Yuan C, Mei W
Antioxidants (Basel, Switzerland) 2025 Aug 6;14(8)

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The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve.

Ma Q, Wu L, Wu J, Ni B, Wang J, Song S
Reproductive sciences (Thousand Oaks, Calif.) 2025 Apr;32(4):1202-1212

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Rubicon, a Key Molecule for Oxidative Stress-Mediated DNA Damage, in Ovarian Granulosa Cells.

Yamada K, Ito M, Nunomura H, Nishigori T, Furuta A, Yoshida M, Yamaki A, Nakamura T, Iwase A, Shima T, Nakashima A
Antioxidants (Basel, Switzerland) 2025 Apr 15;14(4)

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Diltiazem Hydrochloride Protects Against Myocardial Ischemia/Reperfusion Injury in a BNIP3L/NIX-Mediated Mitophagy Manner.

Zhou X, Lu Q, Wang Q, Chu W, Huang J, Yu J, Nong Y, Lu W
Journal of inflammation research 2024;17:8905-8919

---

Deapioplatycodin D promotes cell senescence induced by P21 through the mediation of incomplete mitophagy via BNIP3L.

Li Y, Xiao P, Sun Y, Li Y, Zhao H, Sun J, Wang X, Han X, Jin N, Li X, Bao Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Sep;178:117215

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PI3K p85α/HIF-1α accelerates the development of pulmonary arterial hypertension by regulating fatty acid uptake and mitophagy.

Chen C, Qin S, Song X, Wen J, Huang W, Sheng Z, Li X, Cao Y
Molecular medicine (Cambridge, Mass.) 2024 Nov 11;30(1):208

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Targeting DRP1 with Mdivi-1 to correct mitochondrial abnormalities in ADOA+ syndrome.

Lin Y, Wang D, Li B, Wang J, Xu L, Sun X, Ji K, Yan C, Liu F, Zhao Y
JCI insight 2024 Jun 25;9(15)

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Mechanism Research of DHEA Treatment Improving Diminished Ovarian Reserve by Attenuating the AMPK-SIRT1 Signaling and Mitophagy.

Ma Q, Shen M, Wu J, Ye C, Tan Y
Reproductive sciences (Thousand Oaks, Calif.) 2024 Jul;31(7):2059-2072

---

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells.

Choudhury D, Rong N, Senthil Kumar HV, Swedick S, Samuel RZ, Mehrotra P, Toftegaard J, Rajabian N, Thiyagarajan R, Podder AK, Wu Y, Shahini S, Seldeen KL, Troen B, Lei P, Andreadis ST
Cell reports 2024 Feb 27;43(2):113738

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Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus.

Xiang Q, Wouters C, Chang P, Lu YN, Liu M, Wang H, Yang J, Pekosz A, Zhang Y, Wang J
bioRxiv : the preprint server for biology 2024 Dec 5;

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Bone marrow mesenchymal stem cells modulate miR-202-3p to suppress neuronal apoptosis following spinal cord injury through autophagy activation via the AMPK, MAPK, and PI3K/AKT/mTOR signaling pathway.

Huang K, Fang J, Sun W, Zeng Y, Shi B, Ren B, Bi H, Shuai L
Scientific reports 2024 Dec 3;14(1):30099

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Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1.

Liu Y, Zhang H, Liu Y, Zhang S, Su P, Wang L, Li Y, Liang Y, Wang X, Zhao W, Chen B, Luo D, Zhang N, Yang Q
Autophagy 2023 Sep;19(9):2443-2463

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Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Bai J, Liu T, Tu B, Yuan M, Shu Z, Fan M, Huo S, Guo Y, Wang L, Wang H, Zhao Y
Autophagy 2023 Feb;19(2):632-643

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CoCl(2) -triggered pseudohypoxic stress induces proteasomal degradation of SIRT4 via polyubiquitination of lysines K78 and K299.

Hampel N, Georgy J, Mehrabipour M, Lang A, Lehmkuhl I, Scheller J, Ahmadian MR, Floss DM, Piekorz RP
FEBS open bio 2023 Dec;13(12):2187-2199

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Jionoside A1 alleviates ischemic stroke ischemia/reperfusion injury by promoting Nix-mediated mitophagy.

Yu X, Liu X, Mi X, Luo X, Lian Z, Tang J, Wang G
Cellular and molecular biology (Noisy-le-Grand, France) 2023 Aug 31;69(8):237-245

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Tcap Deficiency in Zebrafish Leads to ROS Production and Mitophagy, and Idebenone Improves its Phenotypes.

Lv X, Zhang R, Xu L, Wang G, Yan C, Lin P
Frontiers in cell and developmental biology 2022;10:836464

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Maintaining homeostasis of mitochondria and endoplasmic reticulum with NSC228155 alleviates cisplatin-induced acute kidney injury.

Li Y, Jiang Y, Zhou W, Wu Y, Zhang S, Ding G, Zhang Y, Zhang A, Huang S, Jia Z, You R
Free radical biology & medicine 2022 Mar;181:270-287

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A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue.

Zhang S, Gong F, Liu J, Liu T, Yang J, Hu J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Jun;150:113004

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In Vivo Metabolic Responses to Different Formulations of Amino Acid Mixtures for the Treatment of Phenylketonuria (PKU).

Giarratana N, Giardino L, Bighinati A, Reiner G, Rocha JC
International journal of molecular sciences 2022 Feb 17;23(4)

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Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy-Lysosome Pathway.

Bao L, Ye J, Liu N, Shao Y, Li W, Fan X, Zhao D, Wang H, Chen X
Molecules (Basel, Switzerland) 2022 Dec 2;27(23)

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Mitochondrial dysfunction and mitophagy pathway activation in hepatitis E virus-infected livers of Mongolian gerbils.

Yang Y, Tian J, Zhang H, Ma M, Li H, Liu T, Yang Y, Liu T, She R
Virus research 2021 Sep;302:198369

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Anti-insulin resistance effects of salidroside through mitochondrial quality control.

You B, Dun Y, Zhang W, Jiang L, Li H, Xie M, Liu Y, Liu S
The Journal of endocrinology 2020 Feb;244(2):383-393

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Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.

Zhang J, Tang N, Zhao Y, Zhao R, Fu X, Zhao D, Zhao Y, Huang L, Li C, Qiu Y, Xue B, Fang L
Journal of proteome research 2020 Apr 3;19(4):1788-1799

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PINK1/Parkin-Mediated Mitophagy Regulation by Reactive Oxygen Species Alleviates Rocaglamide A-Induced Apoptosis in Pancreatic Cancer Cells.

Zhao C, He R, Shen M, Zhu F, Wang M, Liu Y, Chen H, Li X, Qin R
Frontiers in pharmacology 2019;10:968

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Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.

Li H, Ham A, Ma TC, Kuo SH, Kanter E, Kim D, Ko HS, Quan Y, Sardi SP, Li A, Arancio O, Kang UJ, Sulzer D, Tang G
Autophagy 2019 Jan;15(1):113-130

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Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.

Fu T, Xu Z, Liu L, Guo Q, Wu H, Liang X, Zhou D, Xiao L, Liu L, Liu Y, Zhu MS, Chen Q, Gan Z
Cell reports 2018 May 1;23(5):1357-1372

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Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies.

Matsubara S, Bokuda K, Asano Y, Morishima R, Sugaya K, Miyamoto K, Koide R, Komori T, Suzuki S, Nishino I
Neuromuscular disorders : NMD 2018 Mar;28(3):283-288

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Hydrogen Sulphide modulating mitochondrial morphology to promote mitophagy in endothelial cells under high-glucose and high-palmitate.

Liu N, Wu J, Zhang L, Gao Z, Sun Y, Yu M, Zhao Y, Dong S, Lu F, Zhang W
Journal of cellular and molecular medicine 2017 Dec;21(12):3190-3203

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12986-1-AP( BNIP3L Antibody) at dilution of 1:600

Other comments

WB

Appendix

12986-1-AP.pdf

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