- PA5-29385 - Invitrogen Antibodies BNIP3L antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of BNIP3L using 50 µg of mouse liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a BNIP3L polyclonal antibody (Product # PA5-29385) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of BNIP3L using 30 µg of MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a BNIP3L polyclonal antibody (Product # PA5-29385) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of BNIP3L was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a BNIP3L Polyclonal Antibody (Product # PA5-29385) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using BNIP3L Polyclonal Antibody (Product # PA5-29385). Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP3L Polyclonal Antibody (Product # PA5-29385) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-BNIP3L Polyclonal Antibody (Product # PA5-29385) and 35-55 kDa bands corresponding to BNIP3L was observed across bands corresponding to multiple forms of BNIP3L were observed across cell lines tested and was upregulated upon cobalt chloride treatment in HeLa cells. Whole cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), K-562 (Lane 2), HeLa (Lane 3), HeLa treated with cobalt chloride (15 µM for 48 hrs) (Lane 4), SK-BR-3 (Lane 5), HCT 116 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of BNIP3L in methanol-fixed HeLa cells using a BNIP3L polyclonal antibody (Product # PA5-29385) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: BNIP3L Polyclonal Antibody detects BNIP3L protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BNIP3L stained by BNIP3L Polyclonal Antibody (Product # PA5-29385) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BNIP3L (Product # PA5-29385) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-29385