HPA015652

BNIP3L antibody from Atlas Antibodies

BNIP3a, Nix

Eligible for validation within the Antibodypedia Validation Initiative

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

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Antibody Data

Product number

HPA015652

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA015652, RRID:AB_1845406

Product name

Anti-BNIP3L

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDA
QHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTS
RDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRP
ENIPPKEFHFRHPKRSVSLSMRKSGAMK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

MicroRNA-137 Is a Novel Hypoxia-responsive MicroRNA That Inhibits Mitophagy via Regulation of Two Mitophagy Receptors FUNDC1 and NIX
Li W, Zhang X, Zhuang H, Chen H, Chen Y, Tian W, Wu W, Li Y, Wang S, Zhang L, Chen Y, Li L, Zhao B, Sui S, Hu Z, Feng D
Journal of Biological Chemistry 2014 April;289(15):10691-10701

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013 February;10(4):315-323

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human placenta and pancreas tissues using Anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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