Antibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 15010-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15010-1-AP, RRID:AB_2187647
- Product name
- SKP2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SKP2 antibody (Cat. #15010-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CircCRIM1 Promotes Hepatocellular Carcinoma Proliferation and Angiogenesis by Sponging miR-378a-3p and Regulating SKP2 Expression.
MAD2B-mediated cell cycle reentry of podocytes is involved in the pathogenesis of FSGS.
Potential Role of SWI/SNF Complex Subunit Actin-Like Protein 6A in Cervical Cancer.
Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells.
Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.
β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.
USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells.
A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.
Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.
DNAJC5 promotes hepatocellular carcinoma cells proliferation though regulating SKP2 mediated p27 degradation.
p97/VCP is highly expressed in the stem-like cells of breast cancer and controls cancer stemness partly through the unfolded protein response.
Nobiletin downregulates the SKP2-p21/p27-CDK2 axis to inhibit tumor progression and shows synergistic effects with palbociclib on renal cell carcinoma.
The interaction of SKP2 with p27 enhances the progression and stemness of osteosarcoma.
Inhibition of Skp2 Sensitizes Chronic Myeloid Leukemia Cells to Imatinib.
TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.
MAD2B contributes to parietal epithelial cell activation and crescentic glomerulonephritis via Skp2.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Skp2 contributes to cell cycle progression in trophoblast stem cells and to placental development.
Deferasirox protects against hydrogen peroxide-induced cell apoptosis by inhibiting ubiquitination and degradation of p21WAF1/CIP1.
Regulation of circadian rhythms by NEAT1 mediated TMAO-induced endothelial proliferation: A protective role of asparagus extract.
FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.
Long non-coding RNA LINC-01572:28 inhibits granulosa cell growth via a decrease in p27 (Kip1) degradation in patients with polycystic ovary syndrome.
The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy.
The ubiquitin ligase SCFFBXW7α promotes GATA3 degradation.
Rosiglitazone Inhibits Angiotensin II-Induced Proliferation of Glomerular Mesangial Cells via the Gαq/Plcβ4/TRPC Signaling Pathway.
Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.
Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation.
Inhibiting the role of Skp2 suppresses cell proliferation and tumorigenesis of human gastric cancer cells via the upregulation of p27kip1.
MAD2B promotes tubular epithelial-to-mesenchymal transition and renal tubulointerstitial fibrosis via Skp2.
MAD2B contributes to podocyte injury of diabetic nephropathy via inducing cyclin B1 and Skp2 accumulation.
The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.
Ji Y, Yang S, Yan X, Zhu L, Yang W, Yang X, Yu F, Shi L, Zhu X, Lu Y, Zhang C, Lu H, Zhang F
Frontiers in cell and developmental biology 2021;9:796686
Frontiers in cell and developmental biology 2021;9:796686
MAD2B-mediated cell cycle reentry of podocytes is involved in the pathogenesis of FSGS.
Bao D, Su H, Lei CT, Tang H, Ye C, Xiong W, He FF, Lin JH, Hammes HP, Zhang C
International journal of biological sciences 2021;17(15):4396-4408
International journal of biological sciences 2021;17(15):4396-4408
Potential Role of SWI/SNF Complex Subunit Actin-Like Protein 6A in Cervical Cancer.
Wang Q, Cao Z, Wei Y, Zhang J, Cheng Z
Frontiers in oncology 2021;11:724832
Frontiers in oncology 2021;11:724832
Stimulation of ROS Generation by Extract of Warburgia ugandensis Leading to G0/G1 Cell Cycle Arrest and Antiproliferation in A549 Cells.
Zhang YL, Chen GL, Liu Y, Zhuang XC, Guo MQ
Antioxidants (Basel, Switzerland) 2021 Sep 30;10(10)
Antioxidants (Basel, Switzerland) 2021 Sep 30;10(10)
Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.
Zhang J, Zou L, Shi D, Liu J, Zhang J, Zhao R, Wang G, Zhang L, Ouyang L, Liu B
Journal of medicinal chemistry 2021 Oct 14;64(19):14192-14216
Journal of medicinal chemistry 2021 Oct 14;64(19):14192-14216
β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.
Hidalgo-Gutiérrez A, Barriocanal-Casado E, Díaz-Casado ME, González-García P, Zenezini Chiozzi R, Acuña-Castroviejo D, López LC
Biomedicines 2021 Oct 13;9(10)
Biomedicines 2021 Oct 13;9(10)
USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells.
Xia X, Liu X, Chai R, Xu Q, Luo Z, Gu J, Jin Y, Hu T, Yu C, Du B, Huang H, Ou W, Liu S, Liu N
The Journal of biological chemistry 2021 Nov;297(5):101258
The Journal of biological chemistry 2021 Nov;297(5):101258
A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.
Li S, Zhang L, Zhang G, Shangguan G, Hou X, Duan W, Xi Y, Xu N, Zhang B, Dong J, Wang Y, Cui W, Chen S
iScience 2021 Nov 19;24(11):103296
iScience 2021 Nov 19;24(11):103296
Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.
Li J, Chen H, Lou J, Bao G, Wu C, Lou Z, Wang X, Ding J, Li Z, Xiao J, Xu H, Gao W, Zhou K
Journal of cellular physiology 2021 May;236(5):3641-3659
Journal of cellular physiology 2021 May;236(5):3641-3659
DNAJC5 promotes hepatocellular carcinoma cells proliferation though regulating SKP2 mediated p27 degradation.
Wang H, Luo J, Tian X, Xu L, Zhai Z, Cheng M, Chen L, Luo S
Biochimica et biophysica acta. Molecular cell research 2021 May;1868(6):118994
Biochimica et biophysica acta. Molecular cell research 2021 May;1868(6):118994
p97/VCP is highly expressed in the stem-like cells of breast cancer and controls cancer stemness partly through the unfolded protein response.
Li C, Huang Y, Fan Q, Quan H, Dong Y, Nie M, Wang J, Xie F, Ji J, Zhou L, Zheng Z, Wang L
Cell death & disease 2021 Mar 17;12(4):286
Cell death & disease 2021 Mar 17;12(4):286
Nobiletin downregulates the SKP2-p21/p27-CDK2 axis to inhibit tumor progression and shows synergistic effects with palbociclib on renal cell carcinoma.
Chen T, Liu L, Zou Y, Hu X, Zhang W, Zhou T, Luo X, Fu W, Xu J
Cancer biology & medicine 2021 Feb 15;18(1):227-244
Cancer biology & medicine 2021 Feb 15;18(1):227-244
The interaction of SKP2 with p27 enhances the progression and stemness of osteosarcoma.
Wang J, Aldahamsheh O, Ferrena A, Borjihan H, Singla A, Yaguare S, Singh S, Viscarret V, Tingling J, Zi X, Lo Y, Gorlick R, Zheng D, Schwartz EL, Zhao H, Yang R, Geller DS, Hoang BH
Annals of the New York Academy of Sciences 2021 Apr;1490(1):90-104
Annals of the New York Academy of Sciences 2021 Apr;1490(1):90-104
Inhibition of Skp2 Sensitizes Chronic Myeloid Leukemia Cells to Imatinib.
Chen X, Huang Z, Wu W, Xia R
Cancer management and research 2020;12:4777-4787
Cancer management and research 2020;12:4777-4787
TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.
Zhou K, Zheng Z, Li Y, Han W, Zhang J, Mao Y, Chen H, Zhang W, Liu M, Xie L, Zhang H, Xu H, Xiao J
Theranostics 2020;10(20):9280-9302
Theranostics 2020;10(20):9280-9302
MAD2B contributes to parietal epithelial cell activation and crescentic glomerulonephritis via Skp2.
Ye C, Xiong W, Lei CT, Tang H, Su H, Yi F, Zhang C
American journal of physiology. Renal physiology 2020 Oct 1;319(4):F636-F646
American journal of physiology. Renal physiology 2020 Oct 1;319(4):F636-F646
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Li ST, Huang, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269
Nature metabolism 2020 Mar;2(3):256-269
Skp2 contributes to cell cycle progression in trophoblast stem cells and to placental development.
Yamauchi Y, Nita A, Nishiyama M, Muto Y, Shimizu H, Nakatsumi H, Nakayama KI
Genes to cells : devoted to molecular & cellular mechanisms 2020 Jun;25(6):427-438
Genes to cells : devoted to molecular & cellular mechanisms 2020 Jun;25(6):427-438
Deferasirox protects against hydrogen peroxide-induced cell apoptosis by inhibiting ubiquitination and degradation of p21WAF1/CIP1.
Miao J, Xu M, Kuang Y, Pan S, Hou J, Cao P, Duan X, Chang Y, Hasem H, Zhou N, Tan K, Fan Y
Biochemical and biophysical research communications 2020 Apr 9;524(3):736-743
Biochemical and biophysical research communications 2020 Apr 9;524(3):736-743
Regulation of circadian rhythms by NEAT1 mediated TMAO-induced endothelial proliferation: A protective role of asparagus extract.
Wu X, Chen L, Zeb F, Huang Y, An J, Ren J, Yang F, Feng Q
Experimental cell research 2019 Sep 1;382(1):111451
Experimental cell research 2019 Sep 1;382(1):111451
FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.
Zhou K, Chen H, Lin J, Xu H, Wu H, Bao G, Li J, Deng X, Shui X, Gao W, Ding J, Xiao J, Xu H
Cell death & disease 2019 Nov 18;10(12):872
Cell death & disease 2019 Nov 18;10(12):872
Long non-coding RNA LINC-01572:28 inhibits granulosa cell growth via a decrease in p27 (Kip1) degradation in patients with polycystic ovary syndrome.
Zhao J, Xu J, Wang W, Zhao H, Liu H, Liu X, Liu J, Sun Y, Dunaif A, Du Y, Chen ZJ
EBioMedicine 2018 Oct;36:526-538
EBioMedicine 2018 Oct;36:526-538
The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy.
Tsai YC, Kuo PL, Kuo MC, Hung WW, Wu LY, Chang WA, Wu PH, Lee SC, Chen HC, Hsu YL
Journal of clinical medicine 2018 Nov 22;7(12)
Journal of clinical medicine 2018 Nov 22;7(12)
The ubiquitin ligase SCFFBXW7α promotes GATA3 degradation.
Song N, Cao C, Tang Y, Bi L, Jiang Y, Zhou Y, Song X, Liu L, Ge W
Journal of cellular physiology 2018 Mar;233(3):2366-2377
Journal of cellular physiology 2018 Mar;233(3):2366-2377
Rosiglitazone Inhibits Angiotensin II-Induced Proliferation of Glomerular Mesangial Cells via the Gαq/Plcβ4/TRPC Signaling Pathway.
Wei L, Mao J, Lu J, Gao J, Zhu D, Tian L, Chen Z, Jia L, Wang L, Fu R
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(6):2228-2242
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(6):2228-2242
Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.
Zhang S, Chen Q, Liu Q, Li Y, Sun X, Hong L, Ji S, Liu C, Geng J, Zhang W, Lu Z, Yin ZY, Zeng Y, Lin KH, Wu Q, Li Q, Nakayama K, Nakayama KI, Deng X, Johnson RL, Zhu L, Gao D, Chen L, Zhou D
Cancer cell 2017 May 8;31(5):669-684.e7
Cancer cell 2017 May 8;31(5):669-684.e7
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.
Barr AR, Cooper S, Heldt FS, Butera F, Stoy H, Mansfeld J, Novák B, Bakal C
Nature communications 2017 Mar 20;8:14728
Nature communications 2017 Mar 20;8:14728
Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation.
Lanctot AA, Guo Y, Le Y, Edens BM, Nowakowski RS, Feng Y
Cell reports 2017 Aug 1;20(5):1148-1160
Cell reports 2017 Aug 1;20(5):1148-1160
Inhibiting the role of Skp2 suppresses cell proliferation and tumorigenesis of human gastric cancer cells via the upregulation of p27kip1.
Wen Y, Wang K, Yang K
Molecular medicine reports 2016 Oct;14(4):3917-24
Molecular medicine reports 2016 Oct;14(4):3917-24
MAD2B promotes tubular epithelial-to-mesenchymal transition and renal tubulointerstitial fibrosis via Skp2.
Tang H, Fan D, Lei CT, Ye C, Gao P, Chen S, Meng XF, Su H, Zhang C
Journal of molecular medicine (Berlin, Germany) 2016 Nov;94(11):1297-1307
Journal of molecular medicine (Berlin, Germany) 2016 Nov;94(11):1297-1307
MAD2B contributes to podocyte injury of diabetic nephropathy via inducing cyclin B1 and Skp2 accumulation.
Su H, Wan Q, Tian XJ, He FF, Gao P, Tang H, Ye C, Fan D, Chen S, Wang YM, Meng XF, Zhang C
American journal of physiology. Renal physiology 2015 Apr 1;308(7):F728-36
American journal of physiology. Renal physiology 2015 Apr 1;308(7):F728-36
The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.
Wu H, Xiao Y, Zhang S, Ji S, Wei L, Fan F, Geng J, Tian J, Sun X, Qin F, Jin C, Lin J, Yin ZY, Zhang T, Luo L, Li Y, Song S, Lin SC, Deng X, Camargo F, Avruch J, Chen L, Zhou D
Cell reports 2013 May 30;3(5):1663-77
Cell reports 2013 May 30;3(5):1663-77
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15010-1-AP( SKP2 Antibody) at dilution of 1:600
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SKP2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SKP2. This antibody recognizes human,mouse,rat antigen. The SKP2 antibody has been validated for the following applications: ELISA, IHC, WB, IP, IF analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 15010-1-AP(SKP2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 15010-1-AP(SKP2 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue