H00006502-B01P

antibody from Abnova Corporation

Targeting: SKP2 FBL1, FBXL1, p45

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006502-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006502-B01P, RRID:AB_10717746

Product name

SKP2 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human SKP2 protein.

Antigen sequence

MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSG
MGVSALEKEEPDSENIPQELLSNLGHPESPPRKRL
KSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELL
LGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTL
DLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLA
EHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQN
LSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSE
FALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAH
VSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHL
DLSDSVMLKNDCFQEFFQLNYLQHLSLSRCYDIIP
ETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPH
LQINCSHFTTIARPTIGNKKNQEIWGIKCRLTLQK
PSCL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SKP2 expression in transfected 293T cell line (H00006502-T02) by SKP2 MaxPab polyclonal antibody.Lane 1: SKP2 transfected lysate(47.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CDKN1A and SKP2. HeLa cells were stained with anti-CDKN1A rabbit purified polyclonal 1:1200 and anti-SKP2 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)