Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-22037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UFD1L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, H1299, mouse lung. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (91%), Chicken (94%), Rhesus Monkey (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.02 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UFD1L Polyclonal Antibody (Product # PA5-22037). Sample (30 µg of whole cell lysate). A: A431. B: H1299. 10% SDS PAGEUFD1L Polyclonal Antibody (Product # PA5-22037) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UFD1L Polyclonal Antibody detects UFD1L protein by Western blot analysis. A. 50 µg mouse lung lysate/extract.12 % SDS-PAGE. UFD1L Polyclonal Antibody (Product # PA5-22037) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UFD1L in paraformaldehyde-fixed A431 cells using a UFD1L polyclonal antibody (Product # PA5-22037) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using UFD1L antibody (Product # PA5-22037) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of UFD1L was performed in paraffin-embedded mouse kidney tissue using UFD1L Polyclonal Antibody (Product # PA5-22037) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of UFD1L was performed in paraffin-embedded mouse liver tissue using UFD1L Polyclonal Antibody (Product # PA5-22037) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft, using UFD1L (Product # PA5-22037) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.