Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- M01172 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Rabbit IgG Monoclonal antibody for GCN2 detection. Tested positive for WB, IHC, ICC/IF, FC in Human.
- Reactivity
- Human
- Host
- Rabbit
- Antibody clone number
- ACAA-5
- Vial size
- 100ug
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of EIF2AK4 expression in HeLa cell lysate using anti-EIF2AK4 antibody (M01172). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well was loaded with 50ug of HeLa cell lysate under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK4 antigen affinity purified polyclonal antibody (Catalog # M01172) at 0.5 ug/mL overnight at 4
- Additional image