Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NB100-1869AF488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal EAAT1/GLAST-1/SLC1A3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor 488] [NB100-1869AF488] - Intracellular staining of HEK293 cells (1 x 10^6 cells/ml) with EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor® 488] [NB100-1869AF488] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.