PA5-19709

antibody from Invitrogen Antibodies

Targeting: SLC1A3 EA6, EAAT1, GLAST

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

PA5-19709

Provider

Invitrogen Antibodies

Product name

Anti-GLAST Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Not suitable for use with IHC (PFA) or IHC (F). This antibody is predicted to react with rat and cow based on sequence homology.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Bioactive Compound Screen for Pharmacological Enhancers of Apolipoprotein E in Primary Human Astrocytes.

Finan GM, Realubit R, Chung S, Lütjohann D, Wang N, Cirrito JR, Karan C, Kim TW
Cell chemical biology 2016 Dec 22;23(12):1526-1538

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Effects of sleep and wake on astrocytes: clues from molecular and ultrastructural studies.

Bellesi M, de Vivo L, Tononi G, Cirelli C
BMC biology 2015 Aug 25;13:66

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Mouse Brain Tissue Lysate using Product # PA5-19709, EAAT1 primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Mouse Brain Tissue Lysate at a dilution of 2 µg/mL (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-GLAST Polyclonal Antibody (Product # PA519709) and a 55 kDa band corresponding to GLAST was observed across all the tissues tested except Mouse Lung, Mouse Liver and Mouse Kidney. In addition to this, a band at ~130 kDa was also observed which could be the glycosylated form of GLAST. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Lung (Lane 3) Mouse Liver (Lane 4) and Mouse Kidney (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).