TA802482

antibody from OriGene

Targeting: DOT1L DOT1, KIAA1814, KMT4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA802482

Provider

OriGene

Proper citation

OriGene Cat#TA802482, RRID:AB_2626366

Product name

DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)

Antibody type

Monoclonal

Description

DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)

Host

Mouse

Conjugate

Unconjugated

Epitope

DOT1L

Isotype

IgG

Antibody clone number

OTI1D8

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482)

Validation comment

IHC