Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-51960 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Amisyn Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NKKPTQASIT KVKQFEGSTS FVRRSQWMLE QLRQVNGIDP NGDSAEFDLL FENAFDQWVA STASEKCTFF QILHHTCQRY LTDRKPEFIN CQSKIMGGNS ILHSAADSVT SAVQKASQAL NERGERLGRA EEKTEDLK Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Amisyn in control (vector only transfected HEK293T lysate) and STXBP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Amisyn Polyclonal Antibody (Product # PA5-51960).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Amisyn in human cell lines Caco-2 and PC-3 using a Amisyn Polyclonal Antibody (Product # PA5-51960). Corresponding STXBP6 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Amisyn in human lateral ventricle using an Amisyn Polyclonal Antibody (Product # PA5-51960) shows strong positivity in neuronal cells.